Quantitation and cellular localization of 11β-HSD1 expression in murine thymus

Alli M. Nuotio-Antar, Alyssa H. Hasty, William J. Kovacs

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

11β-Hydroxysteroid dehydrogenase type I (11β-HSD1), an NADPH-dependent reductase, functions in intact cells to convert inactive 11-keto metabolites of glucocorticoids into biologically active glucocorticoids. The enzyme is thus capable of amplifying glucocorticoid action in tissues in which it is expressed. In the experiments presented here, we show that 11β-HSD1 is expressed in the murine thymus and that expression increases from late fetal development to maximal levels in the adult thymus. Quantitative real time-PCR, immunoblots, and assays of enzymatic activity reveal adult thymic expression of 11β-HSD1 mRNA and protein at levels approximately 6-7% of those observed in liver. Immunofluorescence experiments show that the enzyme is expressed in the medullary thymocytes and thymocytes present at the corticomedullary junction. These experiments extend our recognition of 11β-HSD1 expression in cells of the immune system and lend support to the notion that glucocorticoid signaling and amplification of those signals by regeneration of active glucocorticoids from inactive 11-keto metabolites might impact intrathymic T cell development and the establishment of the immune repertoire.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume99
Issue number2-3
DOIs
StatePublished - May 1 2006

Fingerprint

Thymus
Thymus Gland
Glucocorticoids
Thymocytes
Metabolites
11-beta-Hydroxysteroid Dehydrogenases
T-cells
Immune system
Experiments
Enzyme Assays
Enzymes
Fetal Development
NADP
Liver
Fluorescent Antibody Technique
Amplification
Real-Time Polymerase Chain Reaction
Regeneration
Immune System
Assays

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{abe7628099b54b91838912ce53dbc4bb,
title = "Quantitation and cellular localization of 11β-HSD1 expression in murine thymus",
abstract = "11β-Hydroxysteroid dehydrogenase type I (11β-HSD1), an NADPH-dependent reductase, functions in intact cells to convert inactive 11-keto metabolites of glucocorticoids into biologically active glucocorticoids. The enzyme is thus capable of amplifying glucocorticoid action in tissues in which it is expressed. In the experiments presented here, we show that 11β-HSD1 is expressed in the murine thymus and that expression increases from late fetal development to maximal levels in the adult thymus. Quantitative real time-PCR, immunoblots, and assays of enzymatic activity reveal adult thymic expression of 11β-HSD1 mRNA and protein at levels approximately 6-7{\%} of those observed in liver. Immunofluorescence experiments show that the enzyme is expressed in the medullary thymocytes and thymocytes present at the corticomedullary junction. These experiments extend our recognition of 11β-HSD1 expression in cells of the immune system and lend support to the notion that glucocorticoid signaling and amplification of those signals by regeneration of active glucocorticoids from inactive 11-keto metabolites might impact intrathymic T cell development and the establishment of the immune repertoire.",
author = "Nuotio-Antar, {Alli M.} and Hasty, {Alyssa H.} and Kovacs, {William J.}",
year = "2006",
month = "5",
day = "1",
doi = "10.1016/j.jsbmb.2006.01.011",
language = "English (US)",
volume = "99",
pages = "93--99",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "2-3",

}

Quantitation and cellular localization of 11β-HSD1 expression in murine thymus. / Nuotio-Antar, Alli M.; Hasty, Alyssa H.; Kovacs, William J.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 99, No. 2-3, 01.05.2006, p. 93-99.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitation and cellular localization of 11β-HSD1 expression in murine thymus

AU - Nuotio-Antar, Alli M.

AU - Hasty, Alyssa H.

AU - Kovacs, William J.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - 11β-Hydroxysteroid dehydrogenase type I (11β-HSD1), an NADPH-dependent reductase, functions in intact cells to convert inactive 11-keto metabolites of glucocorticoids into biologically active glucocorticoids. The enzyme is thus capable of amplifying glucocorticoid action in tissues in which it is expressed. In the experiments presented here, we show that 11β-HSD1 is expressed in the murine thymus and that expression increases from late fetal development to maximal levels in the adult thymus. Quantitative real time-PCR, immunoblots, and assays of enzymatic activity reveal adult thymic expression of 11β-HSD1 mRNA and protein at levels approximately 6-7% of those observed in liver. Immunofluorescence experiments show that the enzyme is expressed in the medullary thymocytes and thymocytes present at the corticomedullary junction. These experiments extend our recognition of 11β-HSD1 expression in cells of the immune system and lend support to the notion that glucocorticoid signaling and amplification of those signals by regeneration of active glucocorticoids from inactive 11-keto metabolites might impact intrathymic T cell development and the establishment of the immune repertoire.

AB - 11β-Hydroxysteroid dehydrogenase type I (11β-HSD1), an NADPH-dependent reductase, functions in intact cells to convert inactive 11-keto metabolites of glucocorticoids into biologically active glucocorticoids. The enzyme is thus capable of amplifying glucocorticoid action in tissues in which it is expressed. In the experiments presented here, we show that 11β-HSD1 is expressed in the murine thymus and that expression increases from late fetal development to maximal levels in the adult thymus. Quantitative real time-PCR, immunoblots, and assays of enzymatic activity reveal adult thymic expression of 11β-HSD1 mRNA and protein at levels approximately 6-7% of those observed in liver. Immunofluorescence experiments show that the enzyme is expressed in the medullary thymocytes and thymocytes present at the corticomedullary junction. These experiments extend our recognition of 11β-HSD1 expression in cells of the immune system and lend support to the notion that glucocorticoid signaling and amplification of those signals by regeneration of active glucocorticoids from inactive 11-keto metabolites might impact intrathymic T cell development and the establishment of the immune repertoire.

UR - http://www.scopus.com/inward/record.url?scp=33646182383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646182383&partnerID=8YFLogxK

U2 - 10.1016/j.jsbmb.2006.01.011

DO - 10.1016/j.jsbmb.2006.01.011

M3 - Article

C2 - 16621520

AN - SCOPUS:33646182383

VL - 99

SP - 93

EP - 99

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 2-3

ER -