Quantitation of adenovirus genome during acute infection in normal children

Hiroko Shike, Chisato Shimizu, John Kanegaye, Jennifer L. Foley, Jane C. Burns

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Adenovirus infection causes a wide range of clinical illness in normal children. New molecular techniques allow quantitation of viral genome to study the natural history of adenovirus infection and viral load in normal children. Methods: Clinical samples were collected from 38 previously healthy, febrile children, and viral cultures were performed. Quantitative polymerase chain reaction (PCR) was used to detect adenovirus genome and to determine viral load. Adenovirus isolates were genotyped with a PCR-based assay. Results: Adenovirus culture was positive in 6 children who were diagnosed with acute adenovirus infection. Throat swabs contained high copy numbers of adenovirus genome (1.6 × 106-6 × 107 copies/swab) from 4 of 4 adenovirus culture-positive children. Only 2 of 32 adenovirus culture-negative children had detectable adenovirus genome from throat swabs, but with a lower copy number (8 × 102 copies/swab). Adenovirus genome was not detected in blood samples from 5 of 6 adenovirus culture-positive children with uncomplicated upper respiratory tract infection and from all adenovirus culture-negative children. High level viremia (1.8 × 10 8/ml) was detected in an adenovirus culture-positive 6-month-old infant with fever, pneumonia, conjunctivitis and hepatitis. Subsequent reduction in viral load paralleled her clinical recovery. Adenovirus viruria (1 × 109 copies/ml) with normal urinanalysis was detected in another adenovirus culture-positive child. All 6 adenovirus isolates were genotyped as adenovirus type 7h. Conclusion: Viral load assessment in clinical samples determined by quantitative PCR can be useful in the diagnosis of adenovirus infection in immunocompetent, febrile children.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalPediatric Infectious Disease Journal
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2005

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Adenoviridae
Genome
Infection
Adenoviridae Infections
Viral Load
Fever
Pharynx
Polymerase Chain Reaction
Conjunctivitis
Viral Genome
Viremia
Respiratory Tract Infections
Hepatitis
Pneumonia

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Shike, Hiroko ; Shimizu, Chisato ; Kanegaye, John ; Foley, Jennifer L. ; Burns, Jane C. / Quantitation of adenovirus genome during acute infection in normal children. In: Pediatric Infectious Disease Journal. 2005 ; Vol. 24, No. 1. pp. 29-33.
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Quantitation of adenovirus genome during acute infection in normal children. / Shike, Hiroko; Shimizu, Chisato; Kanegaye, John; Foley, Jennifer L.; Burns, Jane C.

In: Pediatric Infectious Disease Journal, Vol. 24, No. 1, 01.01.2005, p. 29-33.

Research output: Contribution to journalArticle

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