Quantitative Analysis of Factors Affecting the Event Rate in Glass Nanopore Sensors

Reza Nouri, Zifan Tang, Weihua Guan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

While the solid-state nanopore sensors have shown exceptional promise with their single-molecule sensitivity and label-free operations, one of the most significant challenges in the nanopore sensor is the limited analyte translocation event rate that leads to prolonged sensor response time. This issue is more pronounced when the analyte concentration is below the nanomolar (nM) range, owing to the diffusion-limited mass transport. In this work, we systematically studied the experimental factors beyond the intrinsic analyte concentration and electrophoretic mobility that affect the event rate in glass nanopore sensors. We developed a quantitative model to capture the impact of nanopore surface charge density, ionic strength, nanopore geometry, and translocation direction on the event rate. The synergistic effects of these factors on the event rates were investigated with the aim to find the optimized experimental conditions for operating the glass nanopore sensor from the response time standpoint. The findings in the study would provide useful and practical insight to enhance the device response time and achieve a lower detection limit for various glass nanopore-sensing experiments.

Original languageEnglish (US)
Pages (from-to)3007-3013
Number of pages7
JournalACS Sensors
Volume4
Issue number11
DOIs
StatePublished - Nov 22 2019

Fingerprint

Nanopores
quantitative analysis
Glass
glass
sensors
Sensors
Chemical analysis
Electrophoretic mobility
Intrinsic Factor
Surface charge
Ionic strength
Charge density
solid state
Labels
sensitivity
Mass transfer
geometry
Molecules
molecules
Geometry

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Cite this

Nouri, Reza ; Tang, Zifan ; Guan, Weihua. / Quantitative Analysis of Factors Affecting the Event Rate in Glass Nanopore Sensors. In: ACS Sensors. 2019 ; Vol. 4, No. 11. pp. 3007-3013.
@article{177f2a6beb4c4f27915fec78fbeeaef0,
title = "Quantitative Analysis of Factors Affecting the Event Rate in Glass Nanopore Sensors",
abstract = "While the solid-state nanopore sensors have shown exceptional promise with their single-molecule sensitivity and label-free operations, one of the most significant challenges in the nanopore sensor is the limited analyte translocation event rate that leads to prolonged sensor response time. This issue is more pronounced when the analyte concentration is below the nanomolar (nM) range, owing to the diffusion-limited mass transport. In this work, we systematically studied the experimental factors beyond the intrinsic analyte concentration and electrophoretic mobility that affect the event rate in glass nanopore sensors. We developed a quantitative model to capture the impact of nanopore surface charge density, ionic strength, nanopore geometry, and translocation direction on the event rate. The synergistic effects of these factors on the event rates were investigated with the aim to find the optimized experimental conditions for operating the glass nanopore sensor from the response time standpoint. The findings in the study would provide useful and practical insight to enhance the device response time and achieve a lower detection limit for various glass nanopore-sensing experiments.",
author = "Reza Nouri and Zifan Tang and Weihua Guan",
year = "2019",
month = "11",
day = "22",
doi = "10.1021/acssensors.9b01540",
language = "English (US)",
volume = "4",
pages = "3007--3013",
journal = "ACS Sensors",
issn = "2379-3694",
publisher = "American Chemical Society",
number = "11",

}

Quantitative Analysis of Factors Affecting the Event Rate in Glass Nanopore Sensors. / Nouri, Reza; Tang, Zifan; Guan, Weihua.

In: ACS Sensors, Vol. 4, No. 11, 22.11.2019, p. 3007-3013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative Analysis of Factors Affecting the Event Rate in Glass Nanopore Sensors

AU - Nouri, Reza

AU - Tang, Zifan

AU - Guan, Weihua

PY - 2019/11/22

Y1 - 2019/11/22

N2 - While the solid-state nanopore sensors have shown exceptional promise with their single-molecule sensitivity and label-free operations, one of the most significant challenges in the nanopore sensor is the limited analyte translocation event rate that leads to prolonged sensor response time. This issue is more pronounced when the analyte concentration is below the nanomolar (nM) range, owing to the diffusion-limited mass transport. In this work, we systematically studied the experimental factors beyond the intrinsic analyte concentration and electrophoretic mobility that affect the event rate in glass nanopore sensors. We developed a quantitative model to capture the impact of nanopore surface charge density, ionic strength, nanopore geometry, and translocation direction on the event rate. The synergistic effects of these factors on the event rates were investigated with the aim to find the optimized experimental conditions for operating the glass nanopore sensor from the response time standpoint. The findings in the study would provide useful and practical insight to enhance the device response time and achieve a lower detection limit for various glass nanopore-sensing experiments.

AB - While the solid-state nanopore sensors have shown exceptional promise with their single-molecule sensitivity and label-free operations, one of the most significant challenges in the nanopore sensor is the limited analyte translocation event rate that leads to prolonged sensor response time. This issue is more pronounced when the analyte concentration is below the nanomolar (nM) range, owing to the diffusion-limited mass transport. In this work, we systematically studied the experimental factors beyond the intrinsic analyte concentration and electrophoretic mobility that affect the event rate in glass nanopore sensors. We developed a quantitative model to capture the impact of nanopore surface charge density, ionic strength, nanopore geometry, and translocation direction on the event rate. The synergistic effects of these factors on the event rates were investigated with the aim to find the optimized experimental conditions for operating the glass nanopore sensor from the response time standpoint. The findings in the study would provide useful and practical insight to enhance the device response time and achieve a lower detection limit for various glass nanopore-sensing experiments.

UR - http://www.scopus.com/inward/record.url?scp=85074236254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85074236254&partnerID=8YFLogxK

U2 - 10.1021/acssensors.9b01540

DO - 10.1021/acssensors.9b01540

M3 - Article

C2 - 31612705

AN - SCOPUS:85074236254

VL - 4

SP - 3007

EP - 3013

JO - ACS Sensors

JF - ACS Sensors

SN - 2379-3694

IS - 11

ER -