Quantitative analysis of phospholipids from whey protein concentrates by high-performance liquid chromatography with a narrow-bore column and an evaporative light-scattering detector

M. N. Vaghela, A. Kilara

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A procedure for separation and quantitative determination of phospholipid classes by high-performance liquid chromatography with a narrow-bore column and a light-scattering detector was developed. Cerebrosides (CER), phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylcholine (PC), sphingomeyelin (SPH), and lysophosphatidylcholine (LPC) were base-line resolved, and column life was improved due to low back pressure and low alkalinity of the solvents. Solvent consumption was reduced by 80%, and the detection limit was improved more than tenfold, compared to an analytical column. A gradient elution with pH modifier was necessary for good resolution of acidic phospholipids. A binary solvent system, consisting of A: chloroform/methanol, 80:20 and B: chloroform/methanol/water/ammonium hydroxide (20%), 60:34:6:0.25, was used. Analysis was completed in 36 min, and repeated injections of the samples were possible. The method was applied to the analysis of phospholipids from whey protein concentrates (WPC). Phospholipids in WPC (75% protein) contained (%, w/w) 3.57±0.13 CER; 18.13±1.23 Pl; 4.45±0.21 PE; 7.45±0.58 PS; 30.54±1.84 PC; 35.82±1.16 SPH; and no detectable LPC.

Original languageEnglish (US)
Pages (from-to)729-733
Number of pages5
JournalJournal of the American Oil Chemists' Society
Volume72
Issue number6
DOIs
StatePublished - Jun 1 1995

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Quantitative analysis of phospholipids from whey protein concentrates by high-performance liquid chromatography with a narrow-bore column and an evaporative light-scattering detector'. Together they form a unique fingerprint.

Cite this