Quantitative and qualitative study of STR DNA from ethanol and formalin fixed tissues

Maryam Alqaydi, Reena Roy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Complete and concordant autosomal short tandem repeat (STR) DNA profiles were obtained from 2.0 mg human tissue samples of various types after they were preserved for 24 weeks in 100% ethanol and amplified with the GlobalFiler® and the PowerPlex® Fusion Amplification Kits. When 4.0 mg of the same tissues were preserved for 12 weeks in 10% Neutral Buffered Formalin (NBF) they yielded partial profiles when amplified with the same kits. However, these NBF preserved tissues yielded complete autosomal profiles when amplified with the AmpFlSTR® MiniFiler™ Amplification Kit.Six tissue specimens from the male donor were also amplified with the PowerPlex® Y-23 System. Y-STR profiles were successfully generated from 2.0 mg tissue specimens when preserved for 12 weeks in 100% Ethanol. Only partial profiles were obtained when the fixation time was increased to 24 weeks. Only partial Y-STR profiles were also obtained from 4.0 mg tissue specimen from the same donor when preserved in 10% NBF.In an attempt to optimize the method, the preserved samples that yielded partial profiles were homogenized using the BioMasher III disposable homogenizer and BioMasher III homogenizer and filter. These homogenized tissues did not yield significantly better or more complete profiles when using the GlobalFiler®, AmpFlSTR® MiniFiler™ Amplification Kits, the PowerPlex® Fusion System or the PowerPlex® Y23 System.A total number of 240 tissue samples were analyzed in this project. The amplification of the tissues preserved in 10% NBF with kits such as AmpFlSTR® MiniFiler™ and GlobalFiler® Amplification Kits that contain mini STR primers can be beneficial in forensic testing. The results of the study indicate that in cases such as when a victim or a suspect is missing, the profiles obtained from minute amounts of chemically fixed tissues can be used as reference samples and compared to evidence found at the crime scene.

Original languageEnglish (US)
Pages (from-to)18-29
Number of pages12
JournalForensic Science International
Volume262
DOIs
StatePublished - May 1 2016

Fingerprint

Microsatellite Repeats
Formaldehyde
Ethanol
DNA
Crime

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

@article{c17e581931fd4c4ba1504d6333b3e380,
title = "Quantitative and qualitative study of STR DNA from ethanol and formalin fixed tissues",
abstract = "Complete and concordant autosomal short tandem repeat (STR) DNA profiles were obtained from 2.0 mg human tissue samples of various types after they were preserved for 24 weeks in 100{\%} ethanol and amplified with the GlobalFiler{\circledR} and the PowerPlex{\circledR} Fusion Amplification Kits. When 4.0 mg of the same tissues were preserved for 12 weeks in 10{\%} Neutral Buffered Formalin (NBF) they yielded partial profiles when amplified with the same kits. However, these NBF preserved tissues yielded complete autosomal profiles when amplified with the AmpFlSTR{\circledR} MiniFiler™ Amplification Kit.Six tissue specimens from the male donor were also amplified with the PowerPlex{\circledR} Y-23 System. Y-STR profiles were successfully generated from 2.0 mg tissue specimens when preserved for 12 weeks in 100{\%} Ethanol. Only partial profiles were obtained when the fixation time was increased to 24 weeks. Only partial Y-STR profiles were also obtained from 4.0 mg tissue specimen from the same donor when preserved in 10{\%} NBF.In an attempt to optimize the method, the preserved samples that yielded partial profiles were homogenized using the BioMasher III disposable homogenizer and BioMasher III homogenizer and filter. These homogenized tissues did not yield significantly better or more complete profiles when using the GlobalFiler{\circledR}, AmpFlSTR{\circledR} MiniFiler™ Amplification Kits, the PowerPlex{\circledR} Fusion System or the PowerPlex{\circledR} Y23 System.A total number of 240 tissue samples were analyzed in this project. The amplification of the tissues preserved in 10{\%} NBF with kits such as AmpFlSTR{\circledR} MiniFiler™ and GlobalFiler{\circledR} Amplification Kits that contain mini STR primers can be beneficial in forensic testing. The results of the study indicate that in cases such as when a victim or a suspect is missing, the profiles obtained from minute amounts of chemically fixed tissues can be used as reference samples and compared to evidence found at the crime scene.",
author = "Maryam Alqaydi and Reena Roy",
year = "2016",
month = "5",
day = "1",
doi = "10.1016/j.forsciint.2016.02.011",
language = "English (US)",
volume = "262",
pages = "18--29",
journal = "Forensic Science International",
issn = "0379-0738",
publisher = "Elsevier Ireland Ltd",

}

Quantitative and qualitative study of STR DNA from ethanol and formalin fixed tissues. / Alqaydi, Maryam; Roy, Reena.

In: Forensic Science International, Vol. 262, 01.05.2016, p. 18-29.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative and qualitative study of STR DNA from ethanol and formalin fixed tissues

AU - Alqaydi, Maryam

AU - Roy, Reena

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Complete and concordant autosomal short tandem repeat (STR) DNA profiles were obtained from 2.0 mg human tissue samples of various types after they were preserved for 24 weeks in 100% ethanol and amplified with the GlobalFiler® and the PowerPlex® Fusion Amplification Kits. When 4.0 mg of the same tissues were preserved for 12 weeks in 10% Neutral Buffered Formalin (NBF) they yielded partial profiles when amplified with the same kits. However, these NBF preserved tissues yielded complete autosomal profiles when amplified with the AmpFlSTR® MiniFiler™ Amplification Kit.Six tissue specimens from the male donor were also amplified with the PowerPlex® Y-23 System. Y-STR profiles were successfully generated from 2.0 mg tissue specimens when preserved for 12 weeks in 100% Ethanol. Only partial profiles were obtained when the fixation time was increased to 24 weeks. Only partial Y-STR profiles were also obtained from 4.0 mg tissue specimen from the same donor when preserved in 10% NBF.In an attempt to optimize the method, the preserved samples that yielded partial profiles were homogenized using the BioMasher III disposable homogenizer and BioMasher III homogenizer and filter. These homogenized tissues did not yield significantly better or more complete profiles when using the GlobalFiler®, AmpFlSTR® MiniFiler™ Amplification Kits, the PowerPlex® Fusion System or the PowerPlex® Y23 System.A total number of 240 tissue samples were analyzed in this project. The amplification of the tissues preserved in 10% NBF with kits such as AmpFlSTR® MiniFiler™ and GlobalFiler® Amplification Kits that contain mini STR primers can be beneficial in forensic testing. The results of the study indicate that in cases such as when a victim or a suspect is missing, the profiles obtained from minute amounts of chemically fixed tissues can be used as reference samples and compared to evidence found at the crime scene.

AB - Complete and concordant autosomal short tandem repeat (STR) DNA profiles were obtained from 2.0 mg human tissue samples of various types after they were preserved for 24 weeks in 100% ethanol and amplified with the GlobalFiler® and the PowerPlex® Fusion Amplification Kits. When 4.0 mg of the same tissues were preserved for 12 weeks in 10% Neutral Buffered Formalin (NBF) they yielded partial profiles when amplified with the same kits. However, these NBF preserved tissues yielded complete autosomal profiles when amplified with the AmpFlSTR® MiniFiler™ Amplification Kit.Six tissue specimens from the male donor were also amplified with the PowerPlex® Y-23 System. Y-STR profiles were successfully generated from 2.0 mg tissue specimens when preserved for 12 weeks in 100% Ethanol. Only partial profiles were obtained when the fixation time was increased to 24 weeks. Only partial Y-STR profiles were also obtained from 4.0 mg tissue specimen from the same donor when preserved in 10% NBF.In an attempt to optimize the method, the preserved samples that yielded partial profiles were homogenized using the BioMasher III disposable homogenizer and BioMasher III homogenizer and filter. These homogenized tissues did not yield significantly better or more complete profiles when using the GlobalFiler®, AmpFlSTR® MiniFiler™ Amplification Kits, the PowerPlex® Fusion System or the PowerPlex® Y23 System.A total number of 240 tissue samples were analyzed in this project. The amplification of the tissues preserved in 10% NBF with kits such as AmpFlSTR® MiniFiler™ and GlobalFiler® Amplification Kits that contain mini STR primers can be beneficial in forensic testing. The results of the study indicate that in cases such as when a victim or a suspect is missing, the profiles obtained from minute amounts of chemically fixed tissues can be used as reference samples and compared to evidence found at the crime scene.

UR - http://www.scopus.com/inward/record.url?scp=84959555408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959555408&partnerID=8YFLogxK

U2 - 10.1016/j.forsciint.2016.02.011

DO - 10.1016/j.forsciint.2016.02.011

M3 - Article

C2 - 26963697

AN - SCOPUS:84959555408

VL - 262

SP - 18

EP - 29

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

ER -