Abstract
In purine-depleted environments, the de novo purine biosynthetic pathway is catalyzed to ultimately produce inosine monophosphate (IMP), a purine invisible using current optical microscopy methodology. These enzymes form a complex, termed the 'purinosome,' to replenish IMP levels. Before cellular chemical imaging may be applied to monitor the distributions and fluctuations in purine levels, it is necessary to develop a scheme to quantitatively detect purines. Here, IMP and other purines in biologically relevant matrices have been detected quantitatively. These methods provide a time-of-flight-secondary ion mass spectrometry protocol using C60+ primary ions to determine the concentration of biomolecules in a cell, such as HeLa, at the nanomolar level.
Original language | English (US) |
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Pages (from-to) | 237-239 |
Number of pages | 3 |
Journal | Surface and Interface Analysis |
Volume | 45 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2013 |
All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Condensed Matter Physics
- Surfaces and Interfaces
- Surfaces, Coatings and Films
- Materials Chemistry