In purine-depleted environments, the de novo purine biosynthetic pathway is catalyzed to ultimately produce inosine monophosphate (IMP), a purine invisible using current optical microscopy methodology. These enzymes form a complex, termed the 'purinosome,' to replenish IMP levels. Before cellular chemical imaging may be applied to monitor the distributions and fluctuations in purine levels, it is necessary to develop a scheme to quantitatively detect purines. Here, IMP and other purines in biologically relevant matrices have been detected quantitatively. These methods provide a time-of-flight-secondary ion mass spectrometry protocol using C60+ primary ions to determine the concentration of biomolecules in a cell, such as HeLa, at the nanomolar level.
All Science Journal Classification (ASJC) codes
- Condensed Matter Physics
- Surfaces and Interfaces
- Surfaces, Coatings and Films
- Materials Chemistry