Quantitative detection of purines in biologically relevant films with TOF-Secondary Ion Mass Spectrometry

Lauren M. Jackson, Jonathan J. Hue, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In purine-depleted environments, the de novo purine biosynthetic pathway is catalyzed to ultimately produce inosine monophosphate (IMP), a purine invisible using current optical microscopy methodology. These enzymes form a complex, termed the 'purinosome,' to replenish IMP levels. Before cellular chemical imaging may be applied to monitor the distributions and fluctuations in purine levels, it is necessary to develop a scheme to quantitatively detect purines. Here, IMP and other purines in biologically relevant matrices have been detected quantitatively. These methods provide a time-of-flight-secondary ion mass spectrometry protocol using C60+ primary ions to determine the concentration of biomolecules in a cell, such as HeLa, at the nanomolar level.

Original languageEnglish (US)
Pages (from-to)237-239
Number of pages3
JournalSurface and Interface Analysis
Volume45
Issue number1
DOIs
StatePublished - Jan 2013

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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