Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology