Quantitative determination of urea concentrations in cell culture medium

Robert J.X. Zawada, Peggy Kwan, Kellen L. Olszewski, Manuel Llinas, Shu Gui Huang

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

Original languageEnglish (US)
Pages (from-to)541-544
Number of pages4
JournalBiochemistry and Cell Biology
Volume87
Issue number3
DOIs
StatePublished - Jun 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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