Quantitative point-of-care (POC) assays using measurements of time as the readout

A new type of readout for mHealth

Gregory G. Lewis, Scott T. Phillips

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A paper-based microfluidic device was used to quantitatively detect active enzyme analytes in samples at mid to low femtomolar levels. The device uses a hydrophobic oligomer that controls the wetting properties of the paper within the device. When the target analyte is present within the sample, the oligomer depolymerizes, thus switching the paper to hydrophilic, allowing for the sample to wick through the device. Measuring the time for the sample to wick to a control region relative to an assay region within the device results in sensitive, quantitative measurements of the target enzyme (e.g., alkaline phosphatase or β-d-galactosidase). This device requires the use of only a timer for quantifying a target analyte, and thus the platform may be appropriate for use in resource-limited environments, where access to expensive diagnostic equipment is limited. A smartphone with integrated application software (the software has yet to be developed) could be used for timing the assay and for relating the time measurement to the quantitative readout for the assay. In future versions of this assay, it should be possible to configure the smartphone to start and stop the time-based measurement to further simplify the assay for the user.

Original languageEnglish (US)
Title of host publicationMobile Health Technologies
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages213-229
Number of pages17
ISBN (Electronic)9781493921720
ISBN (Print)9781493921713
DOIs
StatePublished - Jan 27 2015

Fingerprint

Point-of-Care Systems
Telemedicine
Assays
Equipment and Supplies
Capillary Action
Smartphones
Lab-On-A-Chip Devices
Oligomers
Software
Diagnostic Equipment
Galactosidases
Enzymes
Time measurement
Application programs
Microfluidics
Alkaline Phosphatase
Wetting
mHealth

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lewis, G. G., & Phillips, S. T. (2015). Quantitative point-of-care (POC) assays using measurements of time as the readout: A new type of readout for mHealth. In Mobile Health Technologies: Methods and Protocols (pp. 213-229). Springer New York. https://doi.org/10.1007/978-1-4939-2172-0_15
Lewis, Gregory G. ; Phillips, Scott T. / Quantitative point-of-care (POC) assays using measurements of time as the readout : A new type of readout for mHealth. Mobile Health Technologies: Methods and Protocols. Springer New York, 2015. pp. 213-229
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Lewis, GG & Phillips, ST 2015, Quantitative point-of-care (POC) assays using measurements of time as the readout: A new type of readout for mHealth. in Mobile Health Technologies: Methods and Protocols. Springer New York, pp. 213-229. https://doi.org/10.1007/978-1-4939-2172-0_15

Quantitative point-of-care (POC) assays using measurements of time as the readout : A new type of readout for mHealth. / Lewis, Gregory G.; Phillips, Scott T.

Mobile Health Technologies: Methods and Protocols. Springer New York, 2015. p. 213-229.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Lewis GG, Phillips ST. Quantitative point-of-care (POC) assays using measurements of time as the readout: A new type of readout for mHealth. In Mobile Health Technologies: Methods and Protocols. Springer New York. 2015. p. 213-229 https://doi.org/10.1007/978-1-4939-2172-0_15