Quantitative polymerase chain reaction for transforming growth factor-β applied to a field study of fish health in Chesapeake Bay tributaries

Craig A. Harms, Christopher A. Ottinger, Vicki Suzette Blazer, Christine L. Densmore, Laurence H. Pieper, Suzanne Kennedy-Stoskopf

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Fish morbidity and mortality events in Chesapeake Bay tributaries have aroused concern over the health of this important aquatic ecosystem. We applied a recently described method for quantifying mRNA of an immunosuppressive cytokine, transforming growth factor-β (TGF-β), by reverse transcription quantitative-competitive polymerase chain reaction to a field study of fish health in the Chesapeake Basin, and compared the results to those of a traditional cellular immunoassay macrophage bactericidal activity. We selected the white perch (Morone americana) as the sentinel fish species because of its abundance at all of the collection sites. White perch were sampled from Chesapeake Bay tributaries in June, August, and October 1998. Splenic mononuclear cell TGF-β mRNA levels increased and anterior kidney macrophage bactericidal activity decreased, particularly in eastern shore tributaries, from June to August and October. The results of the two assays correlated inversely (Kendall's τb = -0.600; p = 0.0102). The results indicated both temporal and spatial modulation of white perch immune systems in the Chesapeake Basin, and demonstrated the utility of quantitative PCR for TGF-β as a molecular biomarker for field assessment of teleost fish immune status.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalEnvironmental health perspectives
Volume108
Issue number5
StatePublished - Jul 12 2000

Fingerprint

Bass
Transforming Growth Factors
Fishes
Polymerase Chain Reaction
Health
Macrophages
Head Kidney
Messenger RNA
Immunosuppressive Agents
Immunoassay
Reverse Transcription
Ecosystem
Immune System
Biomarkers
Cytokines
Morbidity
Mortality

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Harms, C. A., Ottinger, C. A., Blazer, V. S., Densmore, C. L., Pieper, L. H., & Kennedy-Stoskopf, S. (2000). Quantitative polymerase chain reaction for transforming growth factor-β applied to a field study of fish health in Chesapeake Bay tributaries. Environmental health perspectives, 108(5), 447-452.
Harms, Craig A. ; Ottinger, Christopher A. ; Blazer, Vicki Suzette ; Densmore, Christine L. ; Pieper, Laurence H. ; Kennedy-Stoskopf, Suzanne. / Quantitative polymerase chain reaction for transforming growth factor-β applied to a field study of fish health in Chesapeake Bay tributaries. In: Environmental health perspectives. 2000 ; Vol. 108, No. 5. pp. 447-452.
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Harms, CA, Ottinger, CA, Blazer, VS, Densmore, CL, Pieper, LH & Kennedy-Stoskopf, S 2000, 'Quantitative polymerase chain reaction for transforming growth factor-β applied to a field study of fish health in Chesapeake Bay tributaries', Environmental health perspectives, vol. 108, no. 5, pp. 447-452.

Quantitative polymerase chain reaction for transforming growth factor-β applied to a field study of fish health in Chesapeake Bay tributaries. / Harms, Craig A.; Ottinger, Christopher A.; Blazer, Vicki Suzette; Densmore, Christine L.; Pieper, Laurence H.; Kennedy-Stoskopf, Suzanne.

In: Environmental health perspectives, Vol. 108, No. 5, 12.07.2000, p. 447-452.

Research output: Contribution to journalArticle

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