Quantitative proteomics analysis reveals the signaling networks downstream of EMS1 in the regulation of anther development in Arabidopsis

Jia Nan Lu, Juan Ying Ye, Jun Xu Xu, Hong Ma, Fang Chang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The anthers of angiosperms are organs that produce male gametes, and their normal development is essential for sexual reproduction and plant multiply. The receptor-like kinase EMS1 (EXCESS MICROSPOROCYTES 1) and its extracellular ligand TPD1 (TAPETUM DETERMINANT 1) play important roles in the male fertility by regulating the development of anther tapetum during anther development. However, little is known about the signal transduction network downstream of TPD1-EMS1. With the development of mass spectrometry and isotope labeling technologies, quantitative proteomics has been widely used in plant research and has played an increasingly important role in further studying the molecular phenotype of biological processes and revealing protein regulatory networks. In this paper, iTRAQ-based proteomics analysis was used to compare the proteomic data and transcriptome data of stage 1-9 floral buds from the wild type, ems1 and tpd1 mutants. The results uncovered components of signaling networks, including a large number of proteins with known or unknown functions that they are regulated by TPD1 and EMS1, and provide a new understanding and important reference for further study of the regulation network of anther development.

Original languageEnglish (US)
Pages (from-to)1287-1296
Number of pages10
JournalZhiwu Shengli Xuebao/Plant Physiology Journal
Volume53
Issue number7
DOIs
StatePublished - Jul 20 2017

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All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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