Quantitative study of RNA transmission through ultrafiltration membranes

Ivan Manzano, Andrew Zydney

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nucleic acid-based therapies have the potential to treat a wide range of diseases through their ability to silence and/or regulate gene expression. Strategies for the large-scale purification of RNA are still being developed, creating a need for more fundamental understanding of the behavior of RNA in different separation systems. The objective of this work was to evaluate RNA transmission through a series of composite regenerated cellulose and polyethersulfone ultrafiltration membranes with different molecular weight cutoffs. Data were obtained using Torula yeast RNA, with the size distribution evaluated by both dynamic light scattering and agarose gel electrophoresis. Results with dilute RNA solutions were strongly influenced by RNA adsorption to the composite regenerated cellulose membrane; this effect was not seen with the polyethersulfone membranes. Data for the polyethersulfone membranes were analyzed using the concentration polarization model, which describes the affects of bulk mass transfer on RNA transmission, in combination with available hydrodynamic models for membrane transport. The results provide important insights into the factors controlling RNA transmission and the potential for using ultrafiltration for the purification of nucleic acid therapeutics.

Original languageEnglish (US)
Pages (from-to)272-277
Number of pages6
JournalJournal of Membrane Science
Volume544
DOIs
StatePublished - Jan 1 2017

Fingerprint

Ultrafiltration
RNA
membranes
Membranes
nucleic acids
cellulose
purification
Nucleic acids
Cellulose
Nucleic Acids
composite materials
gene expression
Purification
yeast
electrophoresis
Cryptococcus
mass transfer
Aptitude
therapy
molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Materials Science(all)
  • Physical and Theoretical Chemistry
  • Filtration and Separation

Cite this

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Quantitative study of RNA transmission through ultrafiltration membranes. / Manzano, Ivan; Zydney, Andrew.

In: Journal of Membrane Science, Vol. 544, 01.01.2017, p. 272-277.

Research output: Contribution to journalArticle

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AU - Manzano, Ivan

AU - Zydney, Andrew

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AB - Nucleic acid-based therapies have the potential to treat a wide range of diseases through their ability to silence and/or regulate gene expression. Strategies for the large-scale purification of RNA are still being developed, creating a need for more fundamental understanding of the behavior of RNA in different separation systems. The objective of this work was to evaluate RNA transmission through a series of composite regenerated cellulose and polyethersulfone ultrafiltration membranes with different molecular weight cutoffs. Data were obtained using Torula yeast RNA, with the size distribution evaluated by both dynamic light scattering and agarose gel electrophoresis. Results with dilute RNA solutions were strongly influenced by RNA adsorption to the composite regenerated cellulose membrane; this effect was not seen with the polyethersulfone membranes. Data for the polyethersulfone membranes were analyzed using the concentration polarization model, which describes the affects of bulk mass transfer on RNA transmission, in combination with available hydrodynamic models for membrane transport. The results provide important insights into the factors controlling RNA transmission and the potential for using ultrafiltration for the purification of nucleic acid therapeutics.

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