Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population

Marco Vignuzzi, Jeffrey K. Stone, Jamie J. Arnold, Craig E. Cameron, Raul Andino

Research output: Contribution to journalArticle

651 Scopus citations

Abstract

An RNA virus population does not consist of a single genotype; rather, it is an ensemble of related sequences, termed quasispecies1-4. Quasispecies arise from rapid genomic evolution powered by the high mutation rate of RNA viral replication5-8. Although a high mutation rate is dangerous for a virus because it results in nonviable individuals, it has been hypothesized that high mutation rates create a 'cloud' of potentially beneficial mutations at the population level, which afford the viral quasispecies a greater probability to evolve and adapt to new environments and challenges during infection4,9-11. Mathematical models predict that viral quasispecies are not simply a collection of diverse mutants but a group of interactive variants, which together contribute to the characteristics of the population4,12. According to this view, viral populations, rather than individual variants, are the target of evolutionary selection 4,12. Here we test this hypothesis by examining the consequences of limiting genomic diversity on viral populations. We find that poliovirus carrying a high-fidelity polymerase replicates at wild-type levels but generates less genomic diversity and is unable to adapt to adverse growth conditions. In infected animals, the reduced viral diversity leads to loss of neurotropism and an attenuated pathogenic phenotype. Notably, using chemical mutagenesis to expand quasispecies diversity of the high-fidelity virus before infection restores neurotropism and pathogenesis. Analysis of viruses isolated from brain provides direct evidence for complementation between members in the quasispecies, indicating that selection indeed occurs at the population level rather than on individual variants. Our study provides direct evidence for a fundamental prediction of the quasispecies theory and establishes a link between mutation rate, population dynamics and pathogenesis.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalNature
Volume439
Issue number7074
DOIs
StatePublished - Jan 19 2006

All Science Journal Classification (ASJC) codes

  • General

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    Vignuzzi, M., Stone, J. K., Arnold, J. J., Cameron, C. E., & Andino, R. (2006). Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population. Nature, 439(7074), 344-348. https://doi.org/10.1038/nature04388