Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age

Antiño R. Allen, Kirsten Eilertson, Ayanabha Chakraborti, Sourabh Sharma, Jennifer Baure, Julian Habdank-Kolaczkowski, Barrett Allen, Susanna Rosi, Jacob Raber, John R. Fike

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: Uncontrolled radiation exposure due to radiological terrorism, industrial accidents or military circumstances is a continuing threat for the civilian population. Age plays a major role in the susceptibility to radiation; younger children are at higher risk of developing cognitive deterioration when compared to adults. Our objective was to determine if an exposure to radiation affected the vulnerability of the juvenile hippocampus to a subsequent moderate traumatic injury. Materials and methods: Three-week-old (juvenile) and eight-week-old young adult C57BL/J6 male mice received whole body cesium-137 (137Cs) irradiation with 4 gray (Gy). One month later, unilateral traumatic brain injury was induced using a controlled cortical impact system. Two months post-irradiation, animals were tested for hippocampus-dependent cognitive performance in the Morris water-maze. After cognitive testing, animals were euthanized and their brains frozen for immunohistochemical assessment of activated microglia and neurogenesis in the hippocampal dentate gyrus. Results: All animals were able to learn the water maze task; however, treatment effects were seen when spatial memory retention was assessed. Animals that received irradiation as juveniles followed by a moderate traumatic brain injury one month later did not show spatial memory retention, i.e., were cognitively impaired. In contrast, all groups of animals that were treated as adults showed spatial memory retention in the probe trials. Conclusion: Although the mechanisms involved are not clear, our results suggest that irradiation enhanced a young animal's vulnerability to develop cognitive injury following a subsequent traumatic injury.

Original languageEnglish (US)
Pages (from-to)214-223
Number of pages10
JournalInternational Journal of Radiation Biology
Volume90
Issue number3
DOIs
StatePublished - Mar 2014

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Hippocampus
Wounds and Injuries
Occupational Accidents
Terrorism
Parahippocampal Gyrus
Cesium
Water
Neurogenesis
Dentate Gyrus
Microglia
Traumatic Brain Injury
Radiation Exposure
Young Adult
Radiation
Brain
Population
Retention (Psychology)
Spatial Memory
Therapeutics

All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Allen, A. R., Eilertson, K., Chakraborti, A., Sharma, S., Baure, J., Habdank-Kolaczkowski, J., ... Fike, J. R. (2014). Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age. International Journal of Radiation Biology, 90(3), 214-223. https://doi.org/10.3109/09553002.2014.859761
Allen, Antiño R. ; Eilertson, Kirsten ; Chakraborti, Ayanabha ; Sharma, Sourabh ; Baure, Jennifer ; Habdank-Kolaczkowski, Julian ; Allen, Barrett ; Rosi, Susanna ; Raber, Jacob ; Fike, John R. / Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age. In: International Journal of Radiation Biology. 2014 ; Vol. 90, No. 3. pp. 214-223.
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Allen, AR, Eilertson, K, Chakraborti, A, Sharma, S, Baure, J, Habdank-Kolaczkowski, J, Allen, B, Rosi, S, Raber, J & Fike, JR 2014, 'Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age', International Journal of Radiation Biology, vol. 90, no. 3, pp. 214-223. https://doi.org/10.3109/09553002.2014.859761

Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age. / Allen, Antiño R.; Eilertson, Kirsten; Chakraborti, Ayanabha; Sharma, Sourabh; Baure, Jennifer; Habdank-Kolaczkowski, Julian; Allen, Barrett; Rosi, Susanna; Raber, Jacob; Fike, John R.

In: International Journal of Radiation Biology, Vol. 90, No. 3, 03.2014, p. 214-223.

Research output: Contribution to journalArticle

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T1 - Radiation exposure prior to traumatic brain injury induces responses that differ as a function of animal age

AU - Allen, Antiño R.

AU - Eilertson, Kirsten

AU - Chakraborti, Ayanabha

AU - Sharma, Sourabh

AU - Baure, Jennifer

AU - Habdank-Kolaczkowski, Julian

AU - Allen, Barrett

AU - Rosi, Susanna

AU - Raber, Jacob

AU - Fike, John R.

PY - 2014/3

Y1 - 2014/3

N2 - Purpose: Uncontrolled radiation exposure due to radiological terrorism, industrial accidents or military circumstances is a continuing threat for the civilian population. Age plays a major role in the susceptibility to radiation; younger children are at higher risk of developing cognitive deterioration when compared to adults. Our objective was to determine if an exposure to radiation affected the vulnerability of the juvenile hippocampus to a subsequent moderate traumatic injury. Materials and methods: Three-week-old (juvenile) and eight-week-old young adult C57BL/J6 male mice received whole body cesium-137 (137Cs) irradiation with 4 gray (Gy). One month later, unilateral traumatic brain injury was induced using a controlled cortical impact system. Two months post-irradiation, animals were tested for hippocampus-dependent cognitive performance in the Morris water-maze. After cognitive testing, animals were euthanized and their brains frozen for immunohistochemical assessment of activated microglia and neurogenesis in the hippocampal dentate gyrus. Results: All animals were able to learn the water maze task; however, treatment effects were seen when spatial memory retention was assessed. Animals that received irradiation as juveniles followed by a moderate traumatic brain injury one month later did not show spatial memory retention, i.e., were cognitively impaired. In contrast, all groups of animals that were treated as adults showed spatial memory retention in the probe trials. Conclusion: Although the mechanisms involved are not clear, our results suggest that irradiation enhanced a young animal's vulnerability to develop cognitive injury following a subsequent traumatic injury.

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