Radiation hybrid map of the porcine genome comprising 2035 EST loci

Anette Rink, Katie Eyer, Benjamin Roelofs, Kimberly J. Priest, Kathleen J. Sharkey-Brockmeier, Sudkamon Lekhong, Elif K. Karajusuf, Jason Bang, Martine Yerle, Denis Milan, Wansheng Liu, Craig W. Beattie

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Abstract

The IMpRH7000-rad radiation hybrid panel was used to map 2035 expressed sequence tags (ESTs) at a minimum LOD score of 4.0. A total of 134 linkage groups covers 57,192 cR or 78% of the predicted size of the porcine and 71% of the human genome, respectively. Approximately 81% (1649) of the porcine ESTs were annotated against the NCBI nonredundant database; 1422 mapped in silico to a location in build 35.1 of the human genome sequence (HGS) and 1185 to a gene and location in build 35.1 HGS. The map revealed 40 major breaks in synteny (1.00e -25 and lower) with the human genome, 37 of which fall within a single chromosome. At this improved level of resolution and coverage, porcine chromosomes (SSC) 2, 5, 6, 7, 12, and 14 remain "gene-rich" and homologous to human chromosomes (HSA) 17, 19, and 22, while SSC 1, 8, 11, and X have been confirmed to correspond to the "gene-deserts" on HSA 18, 4, 13, and X.

Original languageEnglish (US)
Pages (from-to)878-885
Number of pages8
JournalMammalian Genome
Volume17
Issue number8
DOIs
StatePublished - Aug 1 2006

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All Science Journal Classification (ASJC) codes

  • Genetics

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Rink, A., Eyer, K., Roelofs, B., Priest, K. J., Sharkey-Brockmeier, K. J., Lekhong, S., Karajusuf, E. K., Bang, J., Yerle, M., Milan, D., Liu, W., & Beattie, C. W. (2006). Radiation hybrid map of the porcine genome comprising 2035 EST loci. Mammalian Genome, 17(8), 878-885. https://doi.org/10.1007/s00335-005-0121-0