Radiation metabolomics. 5. Identification of urinary biomarkers of ionizing radiation exposure in nonhuman primates by mass spectrometry-based metabolomics

Caroline H. Johnson, Andrew David Patterson, Kristopher W. Krausz, John F. Kalinich, John B. Tyburski, Dong Wook Kang, Hans Luecke, Frank J. Gonzalez, William F. Blakely, Jeffrey R. Idle

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Mass spectrometry-based metabolomics has previously demonstrated utility for identifying biomarkers of ionizing radiation exposure in cellular, mouse and rat in vivo radiation models. To provide a valuable link from small laboratory rodents to humans, γ-radiation-induced urinary biomarkers were investigated using a nonhuman primate total-body-irradiation model. Mass spectrometry-based metabolomics approaches were applied to determine whether biomarkers could be identified, as well as the previously discovered rodent biomarkers of γ radiation. Ultra-performance liquid chromatography- electrospray ionization quadrupole time-of-flight mass spectrometry analysis was carried out on a time course of clean-catch urine samples collected from nonhuman primates (n 6 per cohort) exposed to sham, 1.0, 3.5, 6.5 or 8.5 Gy doses of 60Co γ ray (∼0.55 Gy/min) ionizing radiation. By multivariate data analysis, 13 biomarkers of radiation were discovered: N-acetyltaurine, isethionic acid, taurine, xanthine, hypoxanthine, uric acid, creatine, creatinine, tyrosol sulfate, 3-hydroxytyrosol sulfate, tyramine sulfate, N-acetylserotonin sulfate, and adipic acid. N-Acetyltaurine, isethionic acid, and taurine had previously been identified in rats, and taurine and xanthine in mice after ionizing radiation exposure. Mass spectrometry-based metabolomics has thus successfully revealed and verified urinary biomarkers of ionizing radiation exposure in the nonhuman primate for the first time, which indicates possible mechanisms for ionizing radiation injury.

Original languageEnglish (US)
Pages (from-to)328-340
Number of pages13
JournalRadiation Research
Volume178
Issue number4
DOIs
StatePublished - Oct 1 2012

Fingerprint

primates
Metabolomics
biomarkers
radiation dosage
Ionizing Radiation
ionizing radiation
Primates
Mass Spectrometry
mass spectroscopy
Biomarkers
Radiation
Sulfates
Isethionic Acid
sulfates
Taurine
radiation
xanthines
rodents
Xanthine
acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Johnson, Caroline H. ; Patterson, Andrew David ; Krausz, Kristopher W. ; Kalinich, John F. ; Tyburski, John B. ; Kang, Dong Wook ; Luecke, Hans ; Gonzalez, Frank J. ; Blakely, William F. ; Idle, Jeffrey R. / Radiation metabolomics. 5. Identification of urinary biomarkers of ionizing radiation exposure in nonhuman primates by mass spectrometry-based metabolomics. In: Radiation Research. 2012 ; Vol. 178, No. 4. pp. 328-340.
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Johnson, CH, Patterson, AD, Krausz, KW, Kalinich, JF, Tyburski, JB, Kang, DW, Luecke, H, Gonzalez, FJ, Blakely, WF & Idle, JR 2012, 'Radiation metabolomics. 5. Identification of urinary biomarkers of ionizing radiation exposure in nonhuman primates by mass spectrometry-based metabolomics', Radiation Research, vol. 178, no. 4, pp. 328-340. https://doi.org/10.1667/RR2950.1

Radiation metabolomics. 5. Identification of urinary biomarkers of ionizing radiation exposure in nonhuman primates by mass spectrometry-based metabolomics. / Johnson, Caroline H.; Patterson, Andrew David; Krausz, Kristopher W.; Kalinich, John F.; Tyburski, John B.; Kang, Dong Wook; Luecke, Hans; Gonzalez, Frank J.; Blakely, William F.; Idle, Jeffrey R.

In: Radiation Research, Vol. 178, No. 4, 01.10.2012, p. 328-340.

Research output: Contribution to journalArticle

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AU - Kalinich, John F.

AU - Tyburski, John B.

AU - Kang, Dong Wook

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AU - Idle, Jeffrey R.

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