RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato

Tiffany L. Weir, David R. Huff, Barbara J. Christ, C. Peter Romaine

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Random amplified polymorphic DNAPCR (RAPD-PCR) analysis was used to investigate genetic variation among 35 isolates of Alternaria solani, the causal agent of early blight on solanaceous crops, and 30 isolates of A. alternata, an opportunistic pathogen. A symmetric dissimilarity matrix, based on Euclidean metric distances, was used to calculate interpopulation genetic distances (Φ(st)) between the fungal species, geographic origin, and host plant. Of the total genetic variation observed, 34% (Φ(st) = 0.340, P < 0.0001) resided between the two fungal species. A very highly significant interpopulation genetic distance (Φ(st) = 0.2275, P < 0.0001) was observed between isolates of A. solani collected from potato and tomato, suggesting the possibility of pathogenic specialization. There was a significant difference between isolates of A. solani (Φ(st) = 0.1569, P < 0.017) and A. alternata (Φ(st) = 0.1096, P = 0.02) from foreign and domestic sources, but no difference between isolates from Pennsylvania and other states. One primer, P248, generated polymorphisms that could be used to distinguish between the two fungal species.

Original languageEnglish (US)
Pages (from-to)813-821
Number of pages9
JournalMycologia
Volume90
Issue number5
DOIs
StatePublished - 1998

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato'. Together they form a unique fingerprint.

  • Cite this