Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Narasimha V. Hegde, Bhushan M. Jayarao, Chitrita DebRoy

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 103 target cells in a bacterial mixture of 10 5 CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

Original languageEnglish (US)
Pages (from-to)2137-2139
Number of pages3
JournalJournal of clinical microbiology
Volume50
Issue number6
DOIs
StatePublished - Jun 2012

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry'. Together they form a unique fingerprint.

Cite this