Rapid differentiation of Mycobacterium avium and M. paratuberculosis by PCR and restriction enzyme analysis

Inge S. Eriks, Kirsten T. Munck, Thomas E. Besser, Glenn H. Cantor, Vivek Kapur

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Mycobacterium avium subsp. avium (M. avium) and M. avium subsp. paratuberculosis (M. paratuberculosis), intracellular bacteria that can cause chronic granulomatous enteritis in cattle, are difficult to distinguish on the basis of growth and biochemical characteristics. We report the development of a PCR-based strategy for the rapid differentiation of isolates of M. avium from isolates of M. paratuberculosis. Restriction fragment length polymorphism was identified by PCR amplification and subsequent restriction enzyme digestion with PstI of a 960-bp fragment of the 65-kDa heat shock protein (hsp65) from 21 clinical isolates of M. paratuberculosis and 14 isolates of M. avium. These results indicate that a restriction fragment length polymorphism in the hsp65 gene can be used for the rapid differentiation of clinical isolates of M. paratuberculosis and M. avium.

Original languageEnglish (US)
Pages (from-to)734-737
Number of pages4
JournalJournal of clinical microbiology
Volume34
Issue number3
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Rapid differentiation of Mycobacterium avium and M. paratuberculosis by PCR and restriction enzyme analysis'. Together they form a unique fingerprint.

Cite this