Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS

Daniel J. Weston, Diane E. Grotz, Kathleen A. Cox, Jose Castro-Perez, Steve Preece, Richard Tyldesley, Hilary Major, Daniel J. Hayes, Joseph D. Cuiffi, Stephen J. Fonash

Research output: Contribution to conferencePaperpeer-review

Abstract

The use of non-matrix laser desorption methods and MALDI-TOF-mass spectrometry (MS) for rapid drug metabolite profiling was discussed. Optimization of the refined method was carried out on microsomal incubations using nanostructured films in conjunction with a Micromass M@LDI LR instrument, in reflection mode with lock mass correction. The surface of the film was comprised of vertical, rod-like columns, bundled into larger columnar features contained in a continuous void space. The analysis of a microsomal incubation of dextromethorphan showed adequate sensitivity for detection of the parent drug and metabolite.

Original languageEnglish (US)
Pages493-494
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

All Science Journal Classification (ASJC) codes

  • Spectroscopy

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