Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery

Lingling Li, Shirin Munir, John P. Bannantine, Srinand Sreevatsan, Sagarika Kanjilal, Vivek Kapur

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.

Original languageEnglish (US)
Pages (from-to)102-105
Number of pages4
JournalClinical and Vaccine Immunology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Paratuberculosis
Mycobacterium avium
Recombinant Proteins
Antigens
Transcription
Assays
Animals
Ruminants
Proteins
Crohn Disease
Polymerase Chain Reaction
Infection
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Li, Lingling ; Munir, Shirin ; Bannantine, John P. ; Sreevatsan, Srinand ; Kanjilal, Sagarika ; Kapur, Vivek. / Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery. In: Clinical and Vaccine Immunology. 2007 ; Vol. 14, No. 1. pp. 102-105.
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Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery. / Li, Lingling; Munir, Shirin; Bannantine, John P.; Sreevatsan, Srinand; Kanjilal, Sagarika; Kapur, Vivek.

In: Clinical and Vaccine Immunology, Vol. 14, No. 1, 01.01.2007, p. 102-105.

Research output: Contribution to journalArticle

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