Rapid isolation of genomic DNA from animal tissues

G. Konat, G. Gantt, Iwona Hirschler-Laszkiewicz, E. L. Hogan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

Original languageEnglish (US)
Pages (from-to)294-296
Number of pages3
JournalExperimental Cell Research
Volume190
Issue number2
DOIs
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Cell Biology

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