Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes

Yuan Wan, Gong Cheng, Xin Liu, Si Jie Hao, Merisa Nisic, Chuan Dong Zhu, Yi Qiu Xia, Wen Qing Li, Zhi Gang Wang, Wen Long Zhang, Shawn J. Rice, Aswathy Sebastian, Istvan Albert, Chandra P. Belani, Si Yang Zheng

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) can mediate intercellular communication by transferring cargo proteins and nucleic acids between cells. The pathophysiological roles and clinical value of EVs are under intense investigation, yet most studies are limited by technical challenges in the isolation of nanoscale EVs (nEVs). Here, we report a lipid-nanoprobe system that enables spontaneous labelling of nEVs for subsequent magnetic enrichment in 15 minutes, with isolation efficiency and cargo composition similar to what can be achieved by the much slower and bulkier method of ultracentrifugation. We also show that this approach allows for downstream analyses of nucleic acids and proteins, enabling the identification of EGFR and KRAS mutations following nEV isolation from the blood plasma of non-small-cell lung-cancer patients. The efficiency and versatility of the lipid-nanoprobe approach opens up opportunities in point-of-care cancer diagnostics.

Original languageEnglish (US)
Article number0058
JournalNature Biomedical Engineering
Volume1
Issue number4
DOIs
StatePublished - Apr 10 2017

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Nanoprobes
Nucleic acids
Nucleic Acids
Lipids
Proteins
Point-of-Care Systems
Labeling
Blood
Ultracentrifugation
Cells
Plasmas
Non-Small Cell Lung Carcinoma
Communication
Chemical analysis
Mutation
Extracellular Vesicles
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Computer Science Applications

Cite this

Wan, Y., Cheng, G., Liu, X., Hao, S. J., Nisic, M., Zhu, C. D., ... Zheng, S. Y. (2017). Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. Nature Biomedical Engineering, 1(4), [0058]. https://doi.org/10.1038/s41551-017-0058
Wan, Yuan ; Cheng, Gong ; Liu, Xin ; Hao, Si Jie ; Nisic, Merisa ; Zhu, Chuan Dong ; Xia, Yi Qiu ; Li, Wen Qing ; Wang, Zhi Gang ; Zhang, Wen Long ; Rice, Shawn J. ; Sebastian, Aswathy ; Albert, Istvan ; Belani, Chandra P. ; Zheng, Si Yang. / Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. In: Nature Biomedical Engineering. 2017 ; Vol. 1, No. 4.
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Wan, Y, Cheng, G, Liu, X, Hao, SJ, Nisic, M, Zhu, CD, Xia, YQ, Li, WQ, Wang, ZG, Zhang, WL, Rice, SJ, Sebastian, A, Albert, I, Belani, CP & Zheng, SY 2017, 'Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes', Nature Biomedical Engineering, vol. 1, no. 4, 0058. https://doi.org/10.1038/s41551-017-0058

Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. / Wan, Yuan; Cheng, Gong; Liu, Xin; Hao, Si Jie; Nisic, Merisa; Zhu, Chuan Dong; Xia, Yi Qiu; Li, Wen Qing; Wang, Zhi Gang; Zhang, Wen Long; Rice, Shawn J.; Sebastian, Aswathy; Albert, Istvan; Belani, Chandra P.; Zheng, Si Yang.

In: Nature Biomedical Engineering, Vol. 1, No. 4, 0058, 10.04.2017.

Research output: Contribution to journalArticle

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AU - Cheng, Gong

AU - Liu, Xin

AU - Hao, Si Jie

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AU - Zhu, Chuan Dong

AU - Xia, Yi Qiu

AU - Li, Wen Qing

AU - Wang, Zhi Gang

AU - Zhang, Wen Long

AU - Rice, Shawn J.

AU - Sebastian, Aswathy

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AU - Belani, Chandra P.

AU - Zheng, Si Yang

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