Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes

Peter J. Butler, Benjamin P. Bowen, Neal Woodbury

Research output: Contribution to journalConference article

Abstract

Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.

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Endothelial cells
Cell membranes
Endothelial Cells
Cell Membrane
Shear stress
Cytology
Fluorescence Polarization
Hemodynamics
Membrane Lipids
Linings
Photons
Blood Vessels
Cell Biology
Blood
Fluorescence
Health
Polarization

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

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title = "Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes",
abstract = "Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.",
author = "Butler, {Peter J.} and Bowen, {Benjamin P.} and Neal Woodbury",
year = "2002",
month = "12",
day = "1",
language = "English (US)",
volume = "1",
journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",
issn = "1557-170X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

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T1 - Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes

AU - Butler, Peter J.

AU - Bowen, Benjamin P.

AU - Woodbury, Neal

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.

AB - Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.

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M3 - Conference article

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VL - 1

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

SN - 1557-170X

ER -