Raspberry-like assembly of cross-linked nanogels for protein delivery

Urara Hasegawa, Shin ichi Sawada, Takeshi Shimizu, Tsunao Kishida, Eigo Otsuji, Osam Mazda, Kazunari Akiyoshi

Research output: Contribution to journalArticlepeer-review

97 Scopus citations


Raspberry-like assembly of nanogels (A-CHPNG) with a high potential as a carrier for protein delivery was prepared. Cross-linking of acrylate group-modified cholesterol-bearing pullulan nanogel (CHPANG) with thiol group-modified poly (ethylene glycol) (PEGSH) by Michael addition yielded A-CHPNG with narrow size distribution. The size of A-CHPNGs was controlled in the range of 40-120 nm by changing the concentration of CHPANG and PEGSH. A-CHPNG gradually degraded by hydrolysis under physiological condition and seemed to dissociate back to original nanogel. A-CHPNG encapsulated interleukin-12 (IL-12) efficiently (96%) and stably kept it in the presence of BSA (50 mg/ml). In addition, A-CHPNG had a high potential to maintain a high IL-12 level in plasma after subcutaneous injection in mice. Therefore, A-CHPNG is a promising carrier for long-term medications.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalJournal of Controlled Release
Issue number3
StatePublished - Dec 16 2009

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science


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