Rat-brain Na,K-ATPase beta-chain gene

primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.

R. W. Mercer, J. W. Schneider, A. Savitz, J. Emanuel, E. J. Benz, Robert Levenson

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.

Original languageEnglish (US)
Pages (from-to)3884-3890
Number of pages7
JournalMolecular and Cellular Biology
Volume6
Issue number11
DOIs
StatePublished - Jan 1 1986

Fingerprint

Ouabain
HeLa Cells
Brain
Genes
Torpedo
Cardiac Glycosides
Messenger RNA
Amino Acid Sequence
Complementary DNA
RNA
Peptides
sodium-translocating ATPase

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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Rat-brain Na,K-ATPase beta-chain gene : primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells. / Mercer, R. W.; Schneider, J. W.; Savitz, A.; Emanuel, J.; Benz, E. J.; Levenson, Robert.

In: Molecular and Cellular Biology, Vol. 6, No. 11, 01.01.1986, p. 3884-3890.

Research output: Contribution to journalArticle

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AU - Levenson, Robert

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AB - We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.

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