Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: Novel targets for cAMP/cGMP function

Paul A. Kingston, Frank Zufall, Colin Barnstable

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Abstract

Cyclic nucleotide-gated (CNG) channels are Ca2+-permeable, nonspecific cation channels that can be activated through direct interaction with cAMP and/or cGMP. Recent electrophysiological evidence for these channels in cultured hippocampal neurons prompted us to investigate the expression of CNG channel genes in hippocampus. PCR amplification detected the expression of transcripts for subunit 1 of both the rod photoreceptor (RCNGC1) and the olfactory receptor cell (OCNGC1) subtype of CNG channel in adult rat hippocampus. In situ hybridization detected expression of both channel subtypes in most principal neurons, including pyramidal cells of the CA1 through CA3 regions and granule cells of the dentate gyrus. From the hybridization patterns, we conclude that the two genes are colocalized in individual neurons. Comparison of the patterns of expression of type 1 cGMP- dependent protein kinase and the CNG channels suggests that hippocampal neurons can respond to changes in cGMP levels with both rapid changes in CNG channel activity and slower changes induced by phosphorylation. Future models of hippocampal function should include CNG channels and their effects on both electrical responses and intracellular Ca2+ levels.

Original languageEnglish (US)
Pages (from-to)10440-10445
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number19
DOIs
StatePublished - Sep 17 1996

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Cyclic Nucleotide-Gated Cation Channels
Retinal Rod Photoreceptor Cells
Neurons
Genes
Hippocampus
Olfactory Receptor Neurons
Cyclic GMP-Dependent Protein Kinases
Pyramidal Cells
Dentate Gyrus
In Situ Hybridization
Cations
Phosphorylation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "Cyclic nucleotide-gated (CNG) channels are Ca2+-permeable, nonspecific cation channels that can be activated through direct interaction with cAMP and/or cGMP. Recent electrophysiological evidence for these channels in cultured hippocampal neurons prompted us to investigate the expression of CNG channel genes in hippocampus. PCR amplification detected the expression of transcripts for subunit 1 of both the rod photoreceptor (RCNGC1) and the olfactory receptor cell (OCNGC1) subtype of CNG channel in adult rat hippocampus. In situ hybridization detected expression of both channel subtypes in most principal neurons, including pyramidal cells of the CA1 through CA3 regions and granule cells of the dentate gyrus. From the hybridization patterns, we conclude that the two genes are colocalized in individual neurons. Comparison of the patterns of expression of type 1 cGMP- dependent protein kinase and the CNG channels suggests that hippocampal neurons can respond to changes in cGMP levels with both rapid changes in CNG channel activity and slower changes induced by phosphorylation. Future models of hippocampal function should include CNG channels and their effects on both electrical responses and intracellular Ca2+ levels.",
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AU - Zufall, Frank

AU - Barnstable, Colin

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