Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1

K. M. Hannan, R. D. Hannan, S. D. Smith, Leonard "Jim" Jefferson, Mingyue Lun, L. I. Rothblum

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We have previously demonstrated that the protein encoded by the retinoblastoma susceptibility gene (Rb) functions as a regulator of transcription by RNA polymerase I (rDNA transcription) by inhibiting UBF-mediated transcription. In the present study, we have examined the mechanism by which Rb represses UBF-dependent rDNA transcription and determined if other Rb-like proteins have similar effects. We demonstrate that authentic or recombinant UBF and Rb interact directly and this requires a functional A/B pocket. DNase footprinting and band-shift assays demonstrated that the interaction between Rb and UBF does not inhibit the binding of UBF to DNA. However, the formation of an UBF/Rb complex does block the interaction of UBF with SL-1, as indicated by using the 48 kDa subunit as a marker for SL-1. Additional evidence is presented that another pocket protein, p130 but not p107, can be found in a complex with UBF. Interestingly, the cellular content of p130 inversely correlated with the rate of rDNA transcription in two physiological systems, and overexpression of p130 inhibited rDNA transcription. These results suggest that p130 may regulate rDNA transcription in a similar manner to Rb.

Original languageEnglish (US)
Pages (from-to)4988-4999
Number of pages12
JournalOncogene
Volume19
Issue number43
DOIs
StatePublished - Oct 12 2000

Fingerprint

RNA Polymerase I
Ribosomal DNA
Retinoblastoma Genes
Retinoblastoma Protein
Deoxyribonucleases
Proteins
1,2-dihydroxy-4-(nitroethenyl)benzene
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Hannan, K. M. ; Hannan, R. D. ; Smith, S. D. ; Jefferson, Leonard "Jim" ; Lun, Mingyue ; Rothblum, L. I. / Rb and p130 regulate RNA polymerase I transcription : Rb disrupts the interaction between UBF and SL-1. In: Oncogene. 2000 ; Vol. 19, No. 43. pp. 4988-4999.
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Rb and p130 regulate RNA polymerase I transcription : Rb disrupts the interaction between UBF and SL-1. / Hannan, K. M.; Hannan, R. D.; Smith, S. D.; Jefferson, Leonard "Jim"; Lun, Mingyue; Rothblum, L. I.

In: Oncogene, Vol. 19, No. 43, 12.10.2000, p. 4988-4999.

Research output: Contribution to journalArticle

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