43 Citations (Scopus)

Abstract

A native opioid peptide, [Met5]-enkephalin, termed opioid growth factor (OGF), serves as a constitutively expressed and autocrine produced inhibitory molecule related to developing, neoplastic, renewing, and healing tissues. The present study was designed to examine the effects of interfering with opioid-receptor interaction during re-epithelialization of the cornea in the rat using both systemic injections and topical applications of the potent opioid antagonist naltrexone (NTX). A 4 mm diameter epithelial defect was made in the center of the rat cornea. NTX injected twice daily or applied as eyedrops four times daily significantly accelerated re-epithelialization compared to controls. Beginning as early as 8 h after wounding, both the systemic and topical NTX treatment groups bad defects that were approximately 10% to 67% smaller than control abrasions at the time points examined. Similarly, the rate of healing for the NTX groups was 4.7- and 2.8-fold greater than controls for systemic and topical paradigms, respectively. The incidence of complete re-epithelialization in animals given systemic administration of NTX was markedly accelerated in comparison to control rats; however, differences in incidence of repair between NTX and control groups receiving topical application were not observed. These results show that native opioid peptides function in wound healing, and exert a tonically inhibitory influence at the receptor level on repair of corneal epithelial injuries.

Original languageEnglish (US)
Pages (from-to)254-260
Number of pages7
JournalBrain research
Volume798
Issue number1-2
DOIs
StatePublished - Jul 6 1998

Fingerprint

Re-Epithelialization
Naltrexone
Opioid Receptors
Cornea
Opioid Peptides
Narcotic Antagonists
Ophthalmic Solutions
Enkephalins
Incidence
Wound Healing
Opioid Analgesics
Intercellular Signaling Peptides and Proteins
Control Groups
Injections

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

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title = "Re-epithelialization of the rat cornea is accelerated by blockade of opioid receptors",
abstract = "A native opioid peptide, [Met5]-enkephalin, termed opioid growth factor (OGF), serves as a constitutively expressed and autocrine produced inhibitory molecule related to developing, neoplastic, renewing, and healing tissues. The present study was designed to examine the effects of interfering with opioid-receptor interaction during re-epithelialization of the cornea in the rat using both systemic injections and topical applications of the potent opioid antagonist naltrexone (NTX). A 4 mm diameter epithelial defect was made in the center of the rat cornea. NTX injected twice daily or applied as eyedrops four times daily significantly accelerated re-epithelialization compared to controls. Beginning as early as 8 h after wounding, both the systemic and topical NTX treatment groups bad defects that were approximately 10{\%} to 67{\%} smaller than control abrasions at the time points examined. Similarly, the rate of healing for the NTX groups was 4.7- and 2.8-fold greater than controls for systemic and topical paradigms, respectively. The incidence of complete re-epithelialization in animals given systemic administration of NTX was markedly accelerated in comparison to control rats; however, differences in incidence of repair between NTX and control groups receiving topical application were not observed. These results show that native opioid peptides function in wound healing, and exert a tonically inhibitory influence at the receptor level on repair of corneal epithelial injuries.",
author = "Ian Zagon and Joseph Sassani and Patricia McLaughlin",
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Re-epithelialization of the rat cornea is accelerated by blockade of opioid receptors. / Zagon, Ian; Sassani, Joseph; McLaughlin, Patricia.

In: Brain research, Vol. 798, No. 1-2, 06.07.1998, p. 254-260.

Research output: Contribution to journalArticle

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AU - Sassani, Joseph

AU - McLaughlin, Patricia

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