Reaction of cytochrome P450BM3 and peroxynitrite yields nitrosyl complex

Rachel K. Behan, Lee M. Hoffart, Kari L. Stone, Carsten Krebs, Michael T. Green

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Peroxynitrite has come into the spotlight in recent years. Its effects on proteins have been implicated in several diseases such as acute lung injury, rheumatoid arthritis, implant rejection, artherosclerosis, Parkinson's disease, and Alzheimer's disease. Peroxynitrite is thought to inactivate a variety of proteins including thiolate-ligated heme proteins such as cytochrome P450 2B1 and PGI2 synthase, through the nitration of tyrosine residues. In previous studies it was reported that thiolate-ligated heme enzymes react with peroxynitrite to form a ferryl intermediate. In an effort to spectroscopically characterize this species in P450BM3, we discovered that the peroxynitrite-generated intermediate is not an FeIVoxo, but rather an iron-nitrosyl {FeNO}6 complex. We present density functional calculations as well as Mossbauer and stopped-flow spectroscopic characterizations of the peroxynitrite-generated intermediate in P450 BM3.

Original languageEnglish (US)
Pages (from-to)5855-5859
Number of pages5
JournalJournal of the American Chemical Society
Volume129
Issue number18
DOIs
StatePublished - May 9 2007

Fingerprint

Peroxynitrous Acid
Cytochromes
Proteins
Nitration
Density functional theory
Cytochrome P-450 CYP2B1
Hemeproteins
Enzymes
Acute Lung Injury
Iron
Heme
Parkinson Disease
Tyrosine
Rheumatoid Arthritis
Alzheimer Disease

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Behan, Rachel K. ; Hoffart, Lee M. ; Stone, Kari L. ; Krebs, Carsten ; Green, Michael T. / Reaction of cytochrome P450BM3 and peroxynitrite yields nitrosyl complex. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 18. pp. 5855-5859.
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Reaction of cytochrome P450BM3 and peroxynitrite yields nitrosyl complex. / Behan, Rachel K.; Hoffart, Lee M.; Stone, Kari L.; Krebs, Carsten; Green, Michael T.

In: Journal of the American Chemical Society, Vol. 129, No. 18, 09.05.2007, p. 5855-5859.

Research output: Contribution to journalArticle

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