Reactive intermediates in cytochrome P450 catalysis

Courtney M. Krest, Elizabeth L. Onderko, Timothy H. Yosca, Julio C. Calixto, Richard F. Karp, Jovan Livada, Jonathan Rittle, Michael T. Green

Research output: Contribution to journalShort survey

97 Scopus citations

Abstract

Recently, we reported the spectroscopic and kinetic characterizations of cytochrome P450 compound I in CYP119A1, effectively closing the catalytic cycle of cytochrome P450-mediated hydroxylations. In this minireview, we focus on the developments that made this breakthrough possible. We examine the importance of enzyme purification in the quest for reactive intermediates and report the preparation of compound I in a second P450 (P450ST). In an effort to bring clarity to the field, we also examine the validity of controversial reports claiming the production of P450 compound I through the use of peroxynitrite and laser flash photolysis.

Original languageEnglish (US)
Pages (from-to)17074-17081
Number of pages8
JournalJournal of Biological Chemistry
Volume288
Issue number24
DOIs
StatePublished - Jun 14 2013

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Krest, C. M., Onderko, E. L., Yosca, T. H., Calixto, J. C., Karp, R. F., Livada, J., Rittle, J., & Green, M. T. (2013). Reactive intermediates in cytochrome P450 catalysis. Journal of Biological Chemistry, 288(24), 17074-17081. https://doi.org/10.1074/jbc.R113.473108