Real-time characterization of cytotoxicity using single-cell impedance monitoring

Fareid Asphahani, Myo Thein, Kui Wang, David Wood, Sau Shun Wong, Jian Xu, Miqin Zhang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cellular impedance sensors have attracted great attention as a powerful characterization tool for real-time, label-free detection of cytotoxic agents. However, impedance measurements with conventional cell-based sensors that host multiple cells on a single electrode neither provide optimal cell signal sensitivity nor are capable of recording individual cell responses. Here we use a single-cell based platform to monitor cellular impedance on planar microelectrodes to characterize cellular death. In this study, individual cells were selectively patterned on microelectrodes with each hosting one live cell through ligand-mediated natural cell adhesion. Changes in cellular morphology and cell-electrode adherence were monitored after the patterned cells were treated with varying concentrations of hydrogen peroxide, sodium arsenite, and disodium hydrogen arsenate, three potent toxicants related to neurotoxicity and oxidative stress. At low toxicant concentrations, impedance waveforms acquired from individual cells showed variable responses. A time- and concentration-dependent response was seen in the averaged single-cell impedance waveform for all three toxicants. The apoptosis and necrosis characterizations were performed to validate cell impedance results. Furthermore, time constants of apoptosis and necrosis in response to toxicant exposure were analytically established using an equivalent circuit model that characterized the mechanisms of cell death.

Original languageEnglish (US)
Pages (from-to)3011-3019
Number of pages9
JournalAnalyst
Volume137
Issue number13
DOIs
StatePublished - Jul 7 2012

Fingerprint

Microelectrodes
apoptosis
Cell death
Cytotoxicity
Electric Impedance
electrode
Apoptosis
sensor
Electrodes
Oxidative stress
arsenite
Monitoring
Cell adhesion
Cytotoxins
Sensors
arsenate
monitoring
adhesion
Equivalent circuits
hydrogen peroxide

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Asphahani, F., Thein, M., Wang, K., Wood, D., Wong, S. S., Xu, J., & Zhang, M. (2012). Real-time characterization of cytotoxicity using single-cell impedance monitoring. Analyst, 137(13), 3011-3019. https://doi.org/10.1039/c2an16079j
Asphahani, Fareid ; Thein, Myo ; Wang, Kui ; Wood, David ; Wong, Sau Shun ; Xu, Jian ; Zhang, Miqin. / Real-time characterization of cytotoxicity using single-cell impedance monitoring. In: Analyst. 2012 ; Vol. 137, No. 13. pp. 3011-3019.
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Asphahani, F, Thein, M, Wang, K, Wood, D, Wong, SS, Xu, J & Zhang, M 2012, 'Real-time characterization of cytotoxicity using single-cell impedance monitoring', Analyst, vol. 137, no. 13, pp. 3011-3019. https://doi.org/10.1039/c2an16079j

Real-time characterization of cytotoxicity using single-cell impedance monitoring. / Asphahani, Fareid; Thein, Myo; Wang, Kui; Wood, David; Wong, Sau Shun; Xu, Jian; Zhang, Miqin.

In: Analyst, Vol. 137, No. 13, 07.07.2012, p. 3011-3019.

Research output: Contribution to journalArticle

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Asphahani F, Thein M, Wang K, Wood D, Wong SS, Xu J et al. Real-time characterization of cytotoxicity using single-cell impedance monitoring. Analyst. 2012 Jul 7;137(13):3011-3019. https://doi.org/10.1039/c2an16079j