Real-time imaging of cellulose reorientation during cell wall expansion in Arabidopsis roots

Charles T. Anderson, Andrew Carroll, Laila Akhmetova, Chris Somerville

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Cellulose forms the major load-bearing network of the plant cell wall, which simultaneously protects the cell and directs its growth. Although the process of cellulose synthesis has been observed, little is known about the behavior of cellulose in the wall after synthesis. Using Pontamine Fast Scarlet 4B, a dye that fluoresces preferentially in the presence of cellulose and has excitation and emission wavelengths suitable for confocal microscopy, we imaged the architecture and dynamics of cellulose in the cell walls of expanding root cells. We found that cellulose exists in Arabidopsis (Arabidopsis thaliana) cell walls in large fibrillar bundles that vary in orientation. During anisotropic wall expansion in wild-type plants, we observed that these cellulose bundles rotate in a transverse to longitudinal direction. We also found that cellulose organization is significantly altered in mutants lacking either a cellulose synthase subunit or two xyloglucan xylosyltransferase isoforms. Our results support a model in which cellulose is deposited transversely to accommodate longitudinal cell expansion and reoriented during expansion to generate a cell wall that is fortified against strain from any direction.

Original languageEnglish (US)
Pages (from-to)787-796
Number of pages10
JournalPlant physiology
Volume152
Issue number2
DOIs
StatePublished - Feb 1 2010

Fingerprint

Arabidopsis
Cellulose
Cell Wall
cellulose
cell walls
image analysis
cellulose synthase
xyloglucans
synthesis
Weight-Bearing
Plant Cells
cells
Confocal Microscopy
dyes
wavelengths
Protein Isoforms
Coloring Agents
Arabidopsis thaliana
mutants
Growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Anderson, Charles T. ; Carroll, Andrew ; Akhmetova, Laila ; Somerville, Chris. / Real-time imaging of cellulose reorientation during cell wall expansion in Arabidopsis roots. In: Plant physiology. 2010 ; Vol. 152, No. 2. pp. 787-796.
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Real-time imaging of cellulose reorientation during cell wall expansion in Arabidopsis roots. / Anderson, Charles T.; Carroll, Andrew; Akhmetova, Laila; Somerville, Chris.

In: Plant physiology, Vol. 152, No. 2, 01.02.2010, p. 787-796.

Research output: Contribution to journalArticle

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