TY - JOUR
T1 - Real-Time Imaging of Translation on Single mRNA Transcripts in Live Cells
AU - Wang, Chong
AU - Han, Boran
AU - Zhou, Ruobo
AU - Zhuang, Xiaowei
N1 - Funding Information:
We thank Siyuan Wang and Jeff Moffitt for assistance with data analysis and Hazen Babcock for technical support on the imaging setup. This work was supported in part by the Howard Hughes Medical Institute (HHMI). C.W. acknowledges support by the Jane Coffin Childs fellowship. R.Z. acknowledges support by the Life Science Foundation fellowship. X.Z. is a HHMI Investigator.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/5/5
Y1 - 2016/5/5
N2 - Translation is under tight spatial and temporal controls to ensure protein production in the right time and place in cells. Methods that allow real-time, high-resolution visualization of translation in live cells are essential for understanding the spatiotemporal dynamics of translation regulation. Based on multivalent fluorescence amplification of the nascent polypeptide signal, we develop a method to image translation on individual mRNA molecules in real time in live cells, allowing direct visualization of translation events at the translation sites. Using this approach, we monitor transient changes of translation dynamics in responses to environmental stresses, capture distinct mobilities of individual polysomes in different subcellular compartments, and detect 3′ UTR-dependent local translation and active transport of polysomes in dendrites of primary neurons.
AB - Translation is under tight spatial and temporal controls to ensure protein production in the right time and place in cells. Methods that allow real-time, high-resolution visualization of translation in live cells are essential for understanding the spatiotemporal dynamics of translation regulation. Based on multivalent fluorescence amplification of the nascent polypeptide signal, we develop a method to image translation on individual mRNA molecules in real time in live cells, allowing direct visualization of translation events at the translation sites. Using this approach, we monitor transient changes of translation dynamics in responses to environmental stresses, capture distinct mobilities of individual polysomes in different subcellular compartments, and detect 3′ UTR-dependent local translation and active transport of polysomes in dendrites of primary neurons.
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U2 - 10.1016/j.cell.2016.04.040
DO - 10.1016/j.cell.2016.04.040
M3 - Article
C2 - 27153499
AN - SCOPUS:84965146967
SN - 0092-8674
VL - 165
SP - 990
EP - 1001
JO - Cell
JF - Cell
IS - 4
ER -