TY - JOUR
T1 - Real-Time Interaction of Mixed Species Biofilm With Silver Nanoparticles Using QCM-D
AU - Walden, Connie
AU - Greenlee, Lauren
AU - Zhang, Wen
N1 - Funding Information:
Funding: This work was supported by the Engineering Research and Innovation Seed Funding at the University of Arkansas .
Funding Information:
The authors would like to thank Sergio Ivan Perez Bakovic for training and guidance with using the QCM-D instrument. Confocal images were obtained on an instrument provided by the P3 Center, funded through the Arkansas ASSET Initiative II (EPS-1003970) by the National Science Foundation and the Arkansas Science and Technology Authority.
Publisher Copyright:
© 2018
PY - 2019/1
Y1 - 2019/1
N2 - This study presents a novel approach to probe in situ, real-time changes in mixed-species biofilms via quartz crystal microbalance with dissipation (QCM-D). The viscoelasticity of a model wastewater biofilm was monitored by QCM-D, and quantitative changes in both measured frequency, ∆f, and dissipation, ∆D, reflected biofilm structural changes in the presence of silver nanoparticles (Ag-NPs). Complimentary results from confocal laser scanning microscopy confirmed biofilm attachment and the presence of live cells. A step-input experiment with increasing concentrations of Ag-NPs (127, 666, 1160, 1632 ppb) was performed. A Voigt model analysis of QCM-D results shows differing biofilm viscoelastic behavior as a function of Ag-NP concentration. The step input response of ∆D/∆f ratio showed that Ag-NPs cause a structural pattern of firmness at low concentrations (up to 666 ppb), and less rigidity of attached mass at higher concentrations (up to 1632 ppb) for the mixed species biofilm studied.
AB - This study presents a novel approach to probe in situ, real-time changes in mixed-species biofilms via quartz crystal microbalance with dissipation (QCM-D). The viscoelasticity of a model wastewater biofilm was monitored by QCM-D, and quantitative changes in both measured frequency, ∆f, and dissipation, ∆D, reflected biofilm structural changes in the presence of silver nanoparticles (Ag-NPs). Complimentary results from confocal laser scanning microscopy confirmed biofilm attachment and the presence of live cells. A step-input experiment with increasing concentrations of Ag-NPs (127, 666, 1160, 1632 ppb) was performed. A Voigt model analysis of QCM-D results shows differing biofilm viscoelastic behavior as a function of Ag-NP concentration. The step input response of ∆D/∆f ratio showed that Ag-NPs cause a structural pattern of firmness at low concentrations (up to 666 ppb), and less rigidity of attached mass at higher concentrations (up to 1632 ppb) for the mixed species biofilm studied.
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U2 - 10.1016/j.colcom.2018.11.007
DO - 10.1016/j.colcom.2018.11.007
M3 - Article
AN - SCOPUS:85057301940
SN - 2215-0382
VL - 28
SP - 49
EP - 53
JO - Colloids and Interface Science Communications
JF - Colloids and Interface Science Communications
ER -