Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy

Lynn M. DiMemmo, A. Cameron Varano, Jonathan Haulenbeek, Yanping Liang, Kaya Patel, Madeline J. Dukes, Songyan Zheng, Mario Hubert, Steven P. Piccoli, Deborah F. Kelly

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Understanding the properties of protein-based therapeutics is a common goal of biologists and physicians. Technical barriers in the direct observation of small proteins or therapeutic agents can limit our knowledge of how they function in solution and in the body. Electron microscopy (EM) imaging performed in a liquid environment permits us to peer into the active world of cells and molecules at the nanoscale. Here, we employ liquid cell EM to directly visualize a protein-based therapeutic in its native conformation and aggregate state in a time-resolved manner. In combination with quantitative analyses, information from this work contributes new molecular insights toward understanding the behaviours of immunotherapies in a solution state that mimics the human body.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalLab on a Chip
Volume17
Issue number2
DOIs
StatePublished - Jan 21 2017

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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    DiMemmo, L. M., Cameron Varano, A., Haulenbeek, J., Liang, Y., Patel, K., Dukes, M. J., Zheng, S., Hubert, M., Piccoli, S. P., & Kelly, D. F. (2017). Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy. Lab on a Chip, 17(2), 315-322. https://doi.org/10.1039/C6LC01160H