Real-time quantitative PCR in parasitology

Andrew S. Bell, Lisa C. Ranford-Cartwright

Research output: Contribution to journalShort survey

108 Scopus citations

Abstract

Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora. These techniques are truly quantitative, give results over a range of 6-7 orders of magnitude, are quick to perform and require no manipulations post-amplification. They can be used to count genome numbers and to study levels of gene expression. The advantages and limitations of existing thermocyclers and applicable detection systems are discussed here, and promising new developments are highlighted.

Original languageEnglish (US)
Pages (from-to)337-342
Number of pages6
JournalTrends in Parasitology
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2002

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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