Real-Time Visualization of Nanoparticles Interacting with Glioblastoma Stem Cells

Elliot S. Pohlmann, Kaya Patel, Sujuan Guo, Madeline J. Dukes, Zhi Sheng, Deb Kelly

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Nanoparticle-based therapy represents a novel and promising approach to treat glioblastoma, the most common and lethal malignant brain cancer. Although similar therapies have achieved significant cytotoxicity in cultured glioblastoma or glioblastoma stem cells (GSCs), the lack of an appropriate approach to monitor interactions between cells and nanoparticle-based therapies impedes their further clinical application in human patients. To address this critical issue, we first obtained NOTCH1 positive GSCs from patient-derived primary cultures. We then developed a new imaging approach to directly observe the dynamic nature of nanoparticles at the molecular level using in situ transmission electron microscopy (TEM). Utilizing these tools we were able to visualize real-time movements of nanoparticles interacting with GSCs for the first time. Overall, we show strong proof-of-concept results that real-time visualization of nanoparticles in single cells can be achieved at the nanoscale using TEM, thereby providing a powerful platform for the development of nanotherapeutics. (Figured Presented).

Original languageEnglish (US)
Pages (from-to)2329-2335
Number of pages7
JournalNano letters
Volume15
Issue number4
DOIs
StatePublished - Apr 8 2015

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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