Recent and emerging innovations in Salmonella detection: A food and environmental perspective

Rebecca L. Bell, Karen G. Jarvis, Andrea R. Ottesen, Melinda A. Mcfarland, Eric Wayne Brown

Research output: Contribution to journalShort survey

35 Citations (Scopus)

Abstract

Salmonella is a diverse genus of Gram-negative bacilli and a major foodborne pathogen responsible for more than a million illnesses annually in the United States alone. Rapid, reliable detection and identification of this pathogen in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators. Unfortunately, the time to result is too long to effectively monitor foodstuffs, especially those with very short shelf lives. Advances in traditional microbiology and molecular biology over the past 25 years have greatly improved the speed at which this pathogen is detected. Nonetheless, food and environmental samples possess a distinctive set of challenges for these newer, more rapid methodologies. Furthermore, more detailed identification and subtyping strategies still rely heavily on the availability of a pure isolate. However, major shifts in DNA sequencing technologies are meeting this challenge by advancing the detection, identification and subtyping of Salmonella towards a culture-independent diagnostic framework. This review will focus on current approaches and state-of-the-art next-generation advances in the detection, identification and subtyping of Salmonella from food and environmental sources. Rapid, reliable detection and identification of Salmonella in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators; unfortunately the time to result is too long to effectively monitor foodstuffs. This review will focus on current approaches and state-of-the-art next generation advances in molecular biology for the detection, identification, and subtyping of Salmonella from food and environmental sources.

Original languageEnglish (US)
Pages (from-to)279-292
Number of pages14
JournalMicrobial Biotechnology
Volume9
Issue number3
DOIs
StatePublished - May 1 2016

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Salmonella
Innovation
Pathogens
Food
Microbiological Techniques
Food supply
Culture Techniques
Molecular biology
Food Supply
Molecular Biology
Microbiology
Bacilli
DNA Sequence Analysis
Bacillus
DNA
Availability
Technology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Bell, R. L., Jarvis, K. G., Ottesen, A. R., Mcfarland, M. A., & Brown, E. W. (2016). Recent and emerging innovations in Salmonella detection: A food and environmental perspective. Microbial Biotechnology, 9(3), 279-292. https://doi.org/10.1111/1751-7915.12359
Bell, Rebecca L. ; Jarvis, Karen G. ; Ottesen, Andrea R. ; Mcfarland, Melinda A. ; Brown, Eric Wayne. / Recent and emerging innovations in Salmonella detection : A food and environmental perspective. In: Microbial Biotechnology. 2016 ; Vol. 9, No. 3. pp. 279-292.
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Recent and emerging innovations in Salmonella detection : A food and environmental perspective. / Bell, Rebecca L.; Jarvis, Karen G.; Ottesen, Andrea R.; Mcfarland, Melinda A.; Brown, Eric Wayne.

In: Microbial Biotechnology, Vol. 9, No. 3, 01.05.2016, p. 279-292.

Research output: Contribution to journalShort survey

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