Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein

Charles D. Smith, C. Christine Cox, Ralph Snyderman

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

Cleavage of phosphatidylinositol 4,5-bisphosphate by phospholipase C results in the production of two important second messengers: inositol-1,4,5-trisphosphate and 1,2-diacylglycerol. Although several receptors promote this cleavage, the molecular details of phospholipase C activation have remained unresolved. In this study, occupancy of a Ca2+-mobilizing receptor, the oligopeptide chemoattractant receptor on human potymorphonudear leukocyte plasma membranes, was found to lead to the activation of a guanine nucleotide regulatory (N) protein by guanosine 5′-triphosphate. The activated N protein then stimulated a polyphosphoinositide-specific phospholipase C by reducing the Ca2+ requirement for expression of this activity from superphysiological to normal intracellular concentrations. Therefore, the N protein-mediated activation of phospholipase C may be a key step in the pathway of cellular activation by chemoattractants and certain other hormones.

Original languageEnglish (US)
Pages (from-to)97-99
Number of pages3
JournalScience
Volume232
Issue number4746
StatePublished - Jan 1 1986

All Science Journal Classification (ASJC) codes

  • General

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    Smith, C. D., Christine Cox, C., & Snyderman, R. (1986). Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein. Science, 232(4746), 97-99.