Reciprocal regulation of protein tyrosine kinases p56(lck) and p59(fyn), and altered tyrosine phosphorylation in murine AIDS

Mohamed Trebak, Charles A. Lambert, Souad Rahmouni, Roland Greimers, Jacques Boniver, Michel Moutschen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Murine AIDS (MAIDS), caused by a defective murine leukemia virus, is a severe lymphoproliferative disease associated with profound immunodeficiency and increased susceptibility to opportunistic infections. Most subsets of lymphocytes, including CD4+ and CD8+ T cells, are refractory to mitogen stimulation. As a first step to examine proximal signal transduction in the infected mice, Western and Northern blot analyses were performed, and showed that p56(lck) is dramatically decreased at the protein as well as the mRNA level in the lymph nodes (LN). In contrast, p59(fyn) and its mRNA were slightly increased in the LN of the same mice. Similar results were obtained with purified T cells. Interestingly, the thymus of the infected animals did not show any abnormality regarding p56(lck) or p59(fyn). Tyrosine phosphorylation was constitutively increased in the infected mice and was barely amplified by anti-CD3 mAb stimulation. A similar pattern was observed when tyrosine phosphorylation was selectively examined at the level of ZAP-70. Our results suggest that a reciprocal regulation of p56(lck) and p59(fyn) protein tyrosine kinases, previously described in various models of anergy, could also be involved in the pathogenesis of MAIDS.

Original languageEnglish (US)
Pages (from-to)1473-1480
Number of pages8
JournalInternational Immunology
Volume10
Issue number10
DOIs
StatePublished - Oct 14 1998

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Murine Acquired Immunodeficiency Syndrome
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Tyrosine
Phosphorylation
Proto-Oncogene Proteins c-fyn
Lymph Nodes
Defective Viruses
T-Lymphocytes
Murine Leukemia Viruses
Messenger RNA
Opportunistic Infections
Lymphocyte Subsets
Mitogens
Northern Blotting
Thymus Gland
Signal Transduction
Western Blotting
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Trebak, Mohamed ; Lambert, Charles A. ; Rahmouni, Souad ; Greimers, Roland ; Boniver, Jacques ; Moutschen, Michel. / Reciprocal regulation of protein tyrosine kinases p56(lck) and p59(fyn), and altered tyrosine phosphorylation in murine AIDS. In: International Immunology. 1998 ; Vol. 10, No. 10. pp. 1473-1480.
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Reciprocal regulation of protein tyrosine kinases p56(lck) and p59(fyn), and altered tyrosine phosphorylation in murine AIDS. / Trebak, Mohamed; Lambert, Charles A.; Rahmouni, Souad; Greimers, Roland; Boniver, Jacques; Moutschen, Michel.

In: International Immunology, Vol. 10, No. 10, 14.10.1998, p. 1473-1480.

Research output: Contribution to journalArticle

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AU - Trebak, Mohamed

AU - Lambert, Charles A.

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AU - Boniver, Jacques

AU - Moutschen, Michel

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