Recombinant human pp14 inhibits expression of IL-2 by jurkat cells

Na Xiong, Jiannone Mao, Shikha Chakrabortv, Mark L. Tvkocinski

Research output: Contribution to journalArticle

Abstract

Placental protein 14 (PP14) is a glycoprotein produced by cells of the reproductive and hematopoietic systems that possesses antiinflammatory activity. A previous study has suggested that PP14 isolated from amniotic fluid can suppress IL-2 production in mixed lymphocyte cultures. To better define PP14's immunomodulatory potential, we have now produced recombinant human PP14 using a pET22b periplasmic expression vector. The his6-tagged PP14 was purified using Ni2-sepharose chromatography. The periplasmic (nonglycosylated) PP14 was shown to retain the immunoinhibitory function of native PP14 in a two-way mixed-lymphocyte reaction. The immunoinhibition could be reversed with polyclonal anti-PP14 antibody, verifying that the inhibition was mediated by PP14. Bypassing the complexities of peripheral blood mononuclear cell populations, we further showed that the recombinant PP14 could suppress IL-2 production by human Jurkat T cells stimulated with PMA plus anti-CD3 mAb. Hence, the data indicate that periplasmic PP14 can mediate IL-2 suppression and that the suppressive effect can be monitored in a T cell line. The mechanism of the IL-2 modulatory effect is under investigation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Pregnancy Proteins
Jurkat Cells
Interleukin-2
T-cells
Lymphocytes
Periplasmic Proteins
T-Lymphocytes
Hematopoietic System
Agarose Chromatography
Mixed Lymphocyte Culture Test
Amniotic Fluid
Chromatography
Cell culture
Recombinant Proteins
Sepharose
Blood Cells
Glycoproteins
Blood
Anti-Inflammatory Agents
Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Xiong, N., Mao, J., Chakrabortv, S., & Tvkocinski, M. L. (1996). Recombinant human pp14 inhibits expression of IL-2 by jurkat cells. FASEB Journal, 10(6).
Xiong, Na ; Mao, Jiannone ; Chakrabortv, Shikha ; Tvkocinski, Mark L. / Recombinant human pp14 inhibits expression of IL-2 by jurkat cells. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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Xiong, N, Mao, J, Chakrabortv, S & Tvkocinski, ML 1996, 'Recombinant human pp14 inhibits expression of IL-2 by jurkat cells', FASEB Journal, vol. 10, no. 6.

Recombinant human pp14 inhibits expression of IL-2 by jurkat cells. / Xiong, Na; Mao, Jiannone; Chakrabortv, Shikha; Tvkocinski, Mark L.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

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Xiong N, Mao J, Chakrabortv S, Tvkocinski ML. Recombinant human pp14 inhibits expression of IL-2 by jurkat cells. FASEB Journal. 1996 Dec 1;10(6).