Recombinant JC viral DNA: Verification and physical map of prototype

Jonathan D. Martin, Karl H. Brackmann, Brian W. Grinnell, Richard J. Frisque, Duard L. Walker, Maurice Green

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Genomic DNA of the human polyomavirus JC was molecularly cloned from DNA extracted from primary human fetal glial cells infected with prototype (MAD-1) virus and from diseased brain tissue from which JC virus originally was isolated. This report documents that these recombinant MAD-1 DNAs are identical, are prototypical, and may serve as unambiguous references to distinguish the variant DNAs produced by independent isolates of JC virus. Possible ambiguity in other recombinants of MAD-1 DNA is discussed. A new restriction endonuclease cleavage map of MAD-1 DNA was derived for 52 sites cleaved by eight enzymes.

Original languageEnglish (US)
Pages (from-to)70-77
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume109
Issue number1
DOIs
StatePublished - Nov 16 1982

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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