Recombinant ovine interferon-tau (roIFN-τ) modulates lymphocyte subsets, and ovine lentivirus (OvLV)-induced lung pathology

B. Singh, Troy Ott, F. W. Bazer, A. De La Concha-Bermejillo

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Abstract

We evaluated the role of roIFN-τ, a type I IFN, as a modulator of blood mononuclear cell (BMC) phenotypes and lung pathology in non-infected, and OvLV-infected lambs. IFN-τ, the ovine pregnancy recognition signal, has antiviral activity against OvLV and HIV and is less toxic. Similar to HJV, OvLV induces lymphoid interstitial pneumonia. 24 lambs were divided into 4 groups: i) Control; ii) Non-infected+106 antiviral units (AVU) roIFN-τ/kg/day s.c.; iii) 106 TCID50 OvLV intratracheal + placebo; iv) 106 TCID50 OvLV intratracheal +106 AVU roIFN-τ/kg/day s.c. BMCs were labeled every week (0-12) with monoclonal antibodies against CD45, CD4, CD8, γδ, MHC II, CD44 and L-selectin (LS) for flow cytometry. The results were compared within (P≤0.001), and between the groups (P≤0.01) with the Student-Newman-Keuls test. Individually, roIFN-τ and OvLV significantly increased the proportions of all the cell markers except MHC II+ cells in the later. OvLV-infected, roIFN-τ-treated lambs showed higher numbers of CD8+, CD4+ and γδ+ cells than the non-treated, infected lambs. No significant increase was noticed in the infected, treated group for CD44+ and LS+ cells, Histopathology showed lack of lesions in the lungs of roIFN-τ-treated, OvLV-infected as compared to non-treated, infected group. Enhanced cytotoxic cell numbers and a lack of increase in CD44+ and LS+ cells explain the lack of lung pathology in the roIFN-τ-treated, OvLV-infected lambs.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - Dec 1 1997

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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