Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I

Karen Voelkel-Meiman, Ralph Keil, G. Shirleen Roeder

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

A DNA sequence (HOT1) from the repeated ribosomal RNA gene cluster of Saccharomyces cerevisiae can stimulate genetic exchange when inserted at novel locations in the yeast genome. Localization of the sequences required for HOT1 activity demonstrates that two noncontiguous fragments of DNA are required for the stimulation of recombination. One of these fragments contains the transcription initiation site for the major 35S ribosomal RNA precursor. The other contains an enhancer of RNA polymerase I transcription. We suggest that transcription by RNA polymerase I initiating in the inserted rDNA and proceeding through the adjacent sequences is responsible for the stimulation of exchange. Consistent with this interpretation, insertion of the putative termination site for RNA polymerase I transcription between HOT1 and the adjacent recombining DNA abolishes the recombination stimulation. Transcription through both copies of the homologous recombining sequences appears to be necessary for enhanced exchange.

Original languageEnglish (US)
Pages (from-to)1071-1079
Number of pages9
JournalCell
Volume48
Issue number6
DOIs
StatePublished - Mar 27 1987

Fingerprint

RNA Polymerase I
Transcription
Ribosomal DNA
Yeast
Genetic Recombination
Yeasts
Genes
Transcription Initiation Site
DNA
RNA Precursors
Multigene Family
Sequence Homology
rRNA Genes
Ribosomal RNA
Saccharomyces cerevisiae
DNA sequences
Genome

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology

Cite this

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abstract = "A DNA sequence (HOT1) from the repeated ribosomal RNA gene cluster of Saccharomyces cerevisiae can stimulate genetic exchange when inserted at novel locations in the yeast genome. Localization of the sequences required for HOT1 activity demonstrates that two noncontiguous fragments of DNA are required for the stimulation of recombination. One of these fragments contains the transcription initiation site for the major 35S ribosomal RNA precursor. The other contains an enhancer of RNA polymerase I transcription. We suggest that transcription by RNA polymerase I initiating in the inserted rDNA and proceeding through the adjacent sequences is responsible for the stimulation of exchange. Consistent with this interpretation, insertion of the putative termination site for RNA polymerase I transcription between HOT1 and the adjacent recombining DNA abolishes the recombination stimulation. Transcription through both copies of the homologous recombining sequences appears to be necessary for enhanced exchange.",
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Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I. / Voelkel-Meiman, Karen; Keil, Ralph; Roeder, G. Shirleen.

In: Cell, Vol. 48, No. 6, 27.03.1987, p. 1071-1079.

Research output: Contribution to journalArticle

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AU - Keil, Ralph

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