Recombination within the yeast plasmid 2μ circle is site-specific

James Broach, Vicki R. Guarascio, Makkuni Jayaram

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

The multicopy yeast plasmid, 2μ circle, encodes a specialized recombination system. It contains two regions, each 599 bp in length, that are precise inverted repeats of each other and between which recombination occurs readily. In addition, this recombination requires the product of a 2μ circle gene, designated FLP. By examining the products of FLP-mediated recombination of plasmids containing single insertions within one of the repeated regions, we show that this recombination occurs only at a specific site within the repeat. This result was confirmed from analysis of the ability of plasmids containing various deletions within one of the repeated regions to serve as substrates for FLP-mediated recombination. These experiments limit the recombination site to a sequence of less than 65 bp. In addition, by mutational analysis of the recombination potential of a hybrid plasmid containing the entire 2μ circle genome, we have shown that FLP is only the 2μ circle gene necessary for this site-specific recombination. Finally, we describe a sensitive assay for recombination between the repeated sequences of 2μ circle; using it, we demonstrate that even in the absence of FLP gene product, recombination between the repeats occurs at a low but detectable level during meiosis.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalCell
Volume29
Issue number1
DOIs
StatePublished - Jan 1 1982

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Yeast
Genetic Recombination
Plasmids
Genes
Yeasts
Assays
Substrates
Meiosis
Experiments
Genome

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Broach, James ; Guarascio, Vicki R. ; Jayaram, Makkuni. / Recombination within the yeast plasmid 2μ circle is site-specific. In: Cell. 1982 ; Vol. 29, No. 1. pp. 227-234.
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Recombination within the yeast plasmid 2μ circle is site-specific. / Broach, James; Guarascio, Vicki R.; Jayaram, Makkuni.

In: Cell, Vol. 29, No. 1, 01.01.1982, p. 227-234.

Research output: Contribution to journalArticle

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