Abstract

Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalJournal of Molecular Biology
Volume180
Issue number2
DOIs
StatePublished - Dec 5 1984

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Genetic Recombination
Coliphages
Bacteriophages
Nucleotides
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Cheng, Keith ; Smith, Gerald R. / Recombinational hotspot activity of chi-like sequences. In: Journal of Molecular Biology. 1984 ; Vol. 180, No. 2. pp. 371-377.
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title = "Recombinational hotspot activity of chi-like sequences",
abstract = "Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6{\%}, 11{\%} and 38{\%} of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.",
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Recombinational hotspot activity of chi-like sequences. / Cheng, Keith; Smith, Gerald R.

In: Journal of Molecular Biology, Vol. 180, No. 2, 05.12.1984, p. 371-377.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Recombinational hotspot activity of chi-like sequences

AU - Cheng, Keith

AU - Smith, Gerald R.

PY - 1984/12/5

Y1 - 1984/12/5

N2 - Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.

AB - Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.

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