TY - JOUR
T1 - Recombinational hotspot activity of chi-like sequences
AU - Cheng, Keith C.
AU - Smith, Gerald R.
N1 - Funding Information:
We thank Kathy Conklin, Lee Hartwell and Beverly Torok-Storb for helpful comments on the manuscript; we thank Sue Amundsen, Abed Chaudhury, Chris Roberts, Dennis Schultz and Andrew Taylor for helpful discussions and unpublished results as well. This work is dedicated to Dr Michael C. S. Cheng, who passed away 10 February 1984. This research was supported by the NIH through research grant GM-31693 and Research Career Development award AI-00547 (to G.R.S.), and training grant 5 T32 GM-07187 (to K.C.C.).
PY - 1984/12/5
Y1 - 1984/12/5
N2 - Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.
AB - Chi sites, consisting of the nucleotide octamer 5′ G-C-T-G-G-T-G-G 3′, stimulate coliphage λ recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage λ crosses, three of four Chi-like sequences tested, namely 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of λ lacking Chi sites.
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U2 - 10.1016/S0022-2836(84)80009-1
DO - 10.1016/S0022-2836(84)80009-1
M3 - Letter
C2 - 6239928
AN - SCOPUS:0021755950
SN - 0022-2836
VL - 180
SP - 371
EP - 377
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -