Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

Glenn R. Masson, John E. Burke, Natalie G. Ahn, Ganesh S. Anand, Christoph Borchers, Sébastien Brier, George M. Bou-Assaf, John R. Engen, S. Walter Englander, Johan Faber, Rachel Garlish, Patrick R. Griffin, Michael L. Gross, Miklos Guttman, Yoshitomo Hamuro, Albert J.R. Heck, Damian Houde, Roxana E. Iacob, Thomas J.D. Jørgensen, Igor A. KaltashovJudith P. Klinman, Lars Konermann, Petr Man, Leland Mayne, Bruce D. Pascal, Dana Reichmann, Mark Skehel, Joost Snijder, Timothy S. Strutzenberg, Eric S. Underbakke, Cornelia Wagner, Thomas E. Wales, Benjamin T. Walters, David D. Weis, Derek J. Wilson, Patrick L. Wintrode, Zhongqi Zhang, Jie Zheng, David C. Schriemer, Kasper D. Rand

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

Original languageEnglish (US)
Pages (from-to)595-602
Number of pages8
JournalNature methods
Volume16
Issue number7
DOIs
StatePublished - Jul 1 2019

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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