Reconstituting the key steps of the DNA double-strand break repair in vitro.

Matthew J. Rossi, Dmitry V. Bugreev, Olga M. Mazina, Alexander V. Mazin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Double-stranded DNA breaks (DSB), the most harmful type of DNA lesions, cause cell death and genome instability. Homologous recombination repairs DSB using homologous DNA sequences as templates. Here we describe a set of reactions that lead to reconstitution of the double-stranded DNA break repair process in vitro employing purified human homologous recombination proteins and DNA polymerase η. Reconstitution of critical steps of DSB repair in vitro may help to better understand the mechanisms of recombinational DNA repair and the role of various human homologous recombination proteins in this process.

Original languageEnglish (US)
Pages (from-to)407-420
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume745
DOIs
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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