Reconstitution of iron-sulfur center FB results in complete restoration of NADP+ photoreduction in Hg-treated Photosystem I complexes from Synechococcus sp. PCC 6301

Yean Sung Jung, Lian Yu, John H. Golbeck

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46 Scopus citations


The FB iron-sulfur cluster is destroyed preferentially by treating Photosystem I complexes with HgCl2(Kojima Y, Niinomi Y, Tsuboi S, Hiyama T and Sakurai H (1987) Bot Mag 100: 243-53). When FB is 95% depleted but FAis quantitatively retained in cyanobacterial PS I complexes, the reduction potential of FA remains highly electronegative (Em=-530 mV, n=1), the EPR spectral and spin relaxation properties of FA and FXremain unchanged, but NADP+ photoreduction rates decline from 552 to 72 μmol mg Chl-1 h-1.When FB is reconstituted with FeCl3, Na2S and β-mercaptoethanol, NADP+photoreduction rates recover to 528 μmol mg Chl-1 h-1. The correlation between the presence of FBand NADP+ photoreduction provides direct experimental evidence that this iron-sulfur cluster is required for electron throughput from cytochrome c6 to flavodoxin or ferredoxin in Photosystem I.

Original languageEnglish (US)
Pages (from-to)249-255
Number of pages7
JournalPhotosynthesis research
Issue number1-2
StatePublished - Nov 1 1995

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology


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