Reconstitution of the photosystem I secondary quinone acceptor (A1) in the P700-Fx core isolated from synechococcus PCC 6301

Isamu Ikegami, Shigeru Itoh, Patrick G. Warren, John H. Golbeck

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

By treating a FA/FB-depleted P700-Fx core from Synechococcus PCC 6301 with diethylether, most of the phylloquinone was removed without loss of P700. The 1 ms decay of P700+ in the original core was replaced by the 25 ns decay, which was interpreted as the backreaction occurring in a P700+-A0- pair in place of a P700+-Fx- pair. When phylloquinone was added to the phylloquinone-depleted core, the 1 ms decay of P700+ was recovered only in a concomitant presence of Triton X-100. The effective concentration of Triton X-100 was between 0.02% and 0.1%. The flash-induced difference spectrum from 400 to 500 nm confirms the participation of Fx in the 1 ms decay of P700+ in the reconstituted core. Washing the phylloquinone-reconstituted core with n-hexane did not eliminate the effects of phylloquinone, suggesting that the reconstituted phylloquinone was as tightly bound to the P700-Fx core as is the naturally-occurring A1.

Original languageEnglish (US)
Pages (from-to)849-853
Number of pages5
JournalPlant and Cell Physiology
Volume34
Issue number6
DOIs
StatePublished - Sep 1993

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Synechococcus sp. PCC 6301
Vitamin K 1
Synechococcus
Photosystem I Protein Complex
phylloquinone
photosystem I
quinones
deterioration
Octoxynol
hexane
washing
benzoquinone

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

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title = "Reconstitution of the photosystem I secondary quinone acceptor (A1) in the P700-Fx core isolated from synechococcus PCC 6301",
abstract = "By treating a FA/FB-depleted P700-Fx core from Synechococcus PCC 6301 with diethylether, most of the phylloquinone was removed without loss of P700. The 1 ms decay of P700+ in the original core was replaced by the 25 ns decay, which was interpreted as the backreaction occurring in a P700+-A0- pair in place of a P700+-Fx- pair. When phylloquinone was added to the phylloquinone-depleted core, the 1 ms decay of P700+ was recovered only in a concomitant presence of Triton X-100. The effective concentration of Triton X-100 was between 0.02{\%} and 0.1{\%}. The flash-induced difference spectrum from 400 to 500 nm confirms the participation of Fx in the 1 ms decay of P700+ in the reconstituted core. Washing the phylloquinone-reconstituted core with n-hexane did not eliminate the effects of phylloquinone, suggesting that the reconstituted phylloquinone was as tightly bound to the P700-Fx core as is the naturally-occurring A1.",
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Reconstitution of the photosystem I secondary quinone acceptor (A1) in the P700-Fx core isolated from synechococcus PCC 6301. / Ikegami, Isamu; Itoh, Shigeru; Warren, Patrick G.; Golbeck, John H.

In: Plant and Cell Physiology, Vol. 34, No. 6, 09.1993, p. 849-853.

Research output: Contribution to journalArticle

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AU - Golbeck, John H.

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AB - By treating a FA/FB-depleted P700-Fx core from Synechococcus PCC 6301 with diethylether, most of the phylloquinone was removed without loss of P700. The 1 ms decay of P700+ in the original core was replaced by the 25 ns decay, which was interpreted as the backreaction occurring in a P700+-A0- pair in place of a P700+-Fx- pair. When phylloquinone was added to the phylloquinone-depleted core, the 1 ms decay of P700+ was recovered only in a concomitant presence of Triton X-100. The effective concentration of Triton X-100 was between 0.02% and 0.1%. The flash-induced difference spectrum from 400 to 500 nm confirms the participation of Fx in the 1 ms decay of P700+ in the reconstituted core. Washing the phylloquinone-reconstituted core with n-hexane did not eliminate the effects of phylloquinone, suggesting that the reconstituted phylloquinone was as tightly bound to the P700-Fx core as is the naturally-occurring A1.

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